评估用. 的治疗性乙醇叶提取物治疗感染的小鼠的肝脏抗氧化谱

Assessment of Liver Antioxidant Profile in Infected Mice Treated with Curative Ethanol Leaf extract of .

机构信息

Department of Medical Laboratory Science, Rivers State University, Port Harcourt, Nigeria.

Department of Medical Laboratory Science, Madonna University, Elele, Nigeria.

出版信息

Ethiop J Health Sci. 2023 Sep;33(5):761-768. doi: 10.4314/ejhs.v33i5.6.

DOI:10.4314/ejhs.v33i5.6

PMID:38784501

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11111206/

Abstract

BACKGROUND

The increasing resistance to most antimalarial drugs suggests a need for better alternatives. This study evaluated in vivo antimalarial and liver antioxidant profile of dry plantain leaf extract (Musa paradisiaca) on mice infected with Plasmodium berghei.

METHODS

Six groups of ten mice each grouped as control, P. berghei, artesunate, and P. berghei infected mice were orally administered 250,500 and 1000mg/kg Musa paradisiaca leaf extract for 5 days. Blood smears were evaluated for parasitaemia on the 10 day and the mice sacrificed. Catalase, Malondialdehyde, protein, Glutathione peroxidase and reduced glutathione was estimated using Colorimetric, Biuret and spectrophotometric methods respectively with data analyzed using SPSS version 21.

RESULTS

Catalase activity (umol/ml/mins) was 24.62 ± 0.99, 10.04 ± 0.50, 19.35 ± 0.38, 22.13 ± 0.00, 22.79 ± 0.00 and 23.66 ± 0.20 while Glutathione Peroxidase(u/l) was 332.34± 0.64, 205.22± 4.61, 218.26± 0.63, 310.59± 0.00, 305.20± 0.00. and 295.97± 0.02 at Control, P.berghei, artesunate, 250mg, 500mg and1000mg extracts. Glutathione (mM) was 1.60 ± 0.12, 0.64 ± 0.09, 1.06 ± 0.16, 0.72 ± 0.00, 0.92 ± 0.00 and 1.26 ± 0.08 while Malondialdehye (uM) was 16.93 ± 3.59, 61.65 ± 1.72, 27.80 ± 0.26, 36.90 ± 0.00, 34.30 ± 0.00 and 32.68 ± 0.27 and Protein(g/dl) was 22.37 ± 1.87, 7.91 ± 0.13, 11.78 ± 1.19, 11.79 ± 0.00, 13.20 ± 0.00 and 17.04 ±0.03 at control, P.berghei, artesunate, 250mg, 500mg and1000mg respectively.

CONCLUSION

The study suggested that ethanolic extract of Musa paradisiaca reduced liver oxidative stress caused by P.berghei.

摘要

背景

大多数抗疟药物的耐药性不断增加，这表明需要更好的替代品。本研究评估了芭蕉叶（Musa paradisiaca）干粉提取物在感染伯氏疟原虫的小鼠体内的抗疟和肝脏抗氧化特性。

方法

将 6 组每组 10 只的小鼠分为对照组、感染伯氏疟原虫组、青蒿琥酯组和感染伯氏疟原虫组，分别口服 250、500 和 1000mg/kg 的芭蕉叶提取物，连续 5 天。第 10 天评估血涂片疟原虫血症，然后处死小鼠。使用比色法、双缩脲法和分光光度法分别估计过氧化氢酶、丙二醛、蛋白质、谷胱甘肽过氧化物酶和还原型谷胱甘肽，使用 SPSS 版本 21 分析数据。

结果

在对照组、感染伯氏疟原虫组、青蒿琥酯组、250mg 提取物组、500mg 提取物组和 1000mg 提取物组中，过氧化氢酶活性（umol/ml/mins）分别为 24.62 ± 0.99、10.04 ± 0.50、19.35 ± 0.38、22.13 ± 0.00、22.79 ± 0.00 和 23.66 ± 0.20，谷胱甘肽过氧化物酶（u/l）分别为 332.34± 0.64、205.22± 4.61、218.26± 0.63、310.59± 0.00、305.20± 0.00 和 295.97± 0.02，谷胱甘肽（mM）分别为 1.60 ± 0.12、0.64 ± 0.09、1.06 ± 0.16、0.72 ± 0.00、0.92 ± 0.00 和 1.26 ± 0.08，丙二醛（uM）分别为 16.93 ± 3.59、61.65 ± 1.72、27.80 ± 0.26、36.90 ± 0.00、34.30 ± 0.00 和 32.68 ± 0.27，蛋白质（g/dl）分别为 22.37 ± 1.87、7.91 ± 0.13、11.78 ± 1.19、11.79 ± 0.00、13.20 ± 0.00 和 17.04 ±0.03。