基于抗体偶联硼酸亲和识别的磁荧光纳米珠用于超灵敏糖蛋白检测
Integrated magneto-fluorescence nanobeads for ultrasensitive glycoprotein detection using antibody coupled boronate-affinity recognition.
机构信息
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
出版信息
Chem Commun (Camb). 2019 Aug 22;55(69):10312-10315. doi: 10.1039/c9cc04902a.
PMID:31397446
Abstract
Herein, we report a novel magnet-mediated antibody-boronate sandwich-typed assay (ABSTA) strategy for the ultrasensitive, specific, rapid, and enzyme-free detection of glycoproteins in complex samples. The proposed ABSTA method exhibited ultrahigh sensitivity for HCG with a detection limit of 0.19 mIU mL-1, which is approximately 40-fold lower than that of conventional sandwich enzyme immunoassay.
摘要
在此，我们报告了一种新颖的基于磁珠介导的抗体-硼酸酯三明治型测定（ABSTA）策略，用于在复杂样品中进行糖蛋白的超灵敏、特异、快速和无酶检测。所提出的 ABSTA 方法对 HCG 表现出超高的灵敏度，检测限为 0.19 mIU mL-1，大约比传统的夹心酶免疫测定低 40 倍。
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