Pennell C A, Arnold L W, Haughton G, Clarke S H
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
J Immunol. 1988 Oct 15;141(8):2788-96.
The majority of the characterized Ly-1+ B cell lymphomas of B10.H-2aH-4bp/Wts origin (the CH series) bear surface Ig related by Ag specificity or idiotype or both. To determine the genetic basis for these structural similarities, we have sequenced the VH and VL region genes expressed by 10 CH lymphomas, and have compared their VH and V kappa gene rearrangements by Southern blot analysis to one another and to those of four other CH lymphomas. Sequence analysis identified only five different VH, and seven different VL genes, and indicated that these V genes are essentially unmutated. CH lymphomas which express the identical VH gene share at least one idiotope. Thus, the basis for shared idiotype and specificity is due in most cases to the use of the same V gene. This restriction in V gene expression is not due to the preferential use of V genes of any particular VH family or VL group, as the expressed V genes belong to four different VH families and four V kappa groups, and include V lambda 1 and V lambda 2. We hypothesize that Ag selection accounts for the restriction in V gene usage among CH lymphomas.
大多数源自B10.H - 2aH - 4bp/Wts的已鉴定的Ly - 1 + B细胞淋巴瘤（CH系列）带有与抗原特异性或独特型或两者相关的表面免疫球蛋白。为了确定这些结构相似性的遗传基础，我们对10种CH淋巴瘤表达的VH和VL区域基因进行了测序，并通过Southern印迹分析将它们的VH和Vκ基因重排相互比较，并与其他四种CH淋巴瘤的重排进行比较。序列分析仅鉴定出五个不同的VH基因和七个不同的VL基因，并表明这些V基因基本未发生突变。表达相同VH基因的CH淋巴瘤至少共享一个独特位。因此，共享独特型和特异性的基础在大多数情况下是由于使用了相同的V基因。V基因表达的这种限制并非由于优先使用任何特定VH家族或VL组的V基因，因为所表达的V基因属于四个不同的VH家族和四个Vκ组，并且包括Vλ1和Vλ2。我们推测抗原选择是CH淋巴瘤中V基因使用受限的原因。
