支持乳腺退化过程中牛乳蛋白质变化定量分析的蛋白质组学数据。
Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution.
作者信息
Boggs Irina, Hine Brad, Smolenksi Grant, Hettinga Kasper, Zhang Lina, Wheeler Thomas T
机构信息
Dairy Foods, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton 3240, New Zealand.
CSIRO, Agriculture Flagship, Chiswick, Armidale, NSW 2350, Australia.
出版信息
Data Brief. 2016 May 13;8:52-5. doi: 10.1016/j.dib.2016.05.013. eCollection 2016 Sep.
Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic peptides followed by Orbitrap MS/MS (DML), to quantify the changes in the repertoire of bovine milk proteins that occurs after drying off. We analysed skim milk and whey sampled at day 0 and either day 3 or day 8 after drying off. These analyses identified 45 spots by MALDI-TOF, 51 proteins by GeLC and 161 proteins by DML, for which the detailed data work-up is presented as three Excel files. The data supplied in this article supports the accompanying publication "Changes in the repertoire of bovine milk proteins during mammary involution" (Boggs et al., 2015) [1]. Data are available via ProteomeXchange with identifiers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011.
在此，我们提供了来自三种蛋白质组学技术的数据；二维电泳（2-DE），随后使用PSD MALDI-TOF MS/MS鉴定选定的斑点，一维凝胶电泳，随后对凝胶切片进行LC-MS/MS分析（GeLC），以及对胰蛋白酶肽进行二甲基同位素标记，随后进行Orbitrap MS/MS（DML），以量化干奶后牛乳蛋白质库中的变化。我们分析了在第0天以及干奶后第3天或第8天采集的脱脂乳和乳清。这些分析通过MALDI-TOF鉴定出45个斑点，通过GeLC鉴定出51种蛋白质，通过DML鉴定出161种蛋白质，其详细的数据处理以三个Excel文件的形式呈现。本文提供的数据支持了随附的出版物《乳腺退化过程中牛乳蛋白质库的变化》（Boggs等人，2015年）[1]。数据可通过蛋白质组交换获得，标识符为蛋白质组交换：PXD003110和蛋白质组交换：PXD003011。
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