Department of Anatomy, Nantong University Medical School, Jiangsu 226001, China.
Department of Anatomy, Nantong University Medical School, Jiangsu 226001, China.
Cytokine. 2020 Jan;125:154771. doi: 10.1016/j.cyto.2019.154771. Epub 2019 Aug 7.
To investigate the effect of CXCL12 on regeneration of radial glia like cells after traumatic brain injury (TBI). We randomly divided 48 rats into 4 groups: (1) the sham group, rats were performed craniotomy only, (2) the control group, saline were injected into the ipsilateral cortex after TBI, (3) the CXCL12 group, CXCL12 were injected, and (4) the CXCL12 + AMD3100 group, a mixture of CXCL12 and AMD3100 were injected. Seven days after TBI, the brain tissues were subjected to immunofluorescence double-labeled staining of BrdU/Nestin, BLBP/Nestin, BLBP/Vimentin, BLBP/SOX2, BLBP/CXCR4, BLBP/DCX. Western Blot assay was used to measure the levels of Nestin, BLBP, and Vimentin. Compared with the control group, CXCL12 treatment significantly increased the number of cells stained with BrdU/Nestin, BLBP/Nestin, and BLBP/Vimentin around the injured cortex and corpus callosum areas. CXCL12 + AMD3100 treatment significantly decreased the number of these cells compared with the CXCL12 treatment and control group. The protein levels of Nestin, BLBP, and Vimentin had the same change trends as those of the immunofluorescence staining. The BLBP/Vimentin positive cells presented with the astrocyte pattern around the injured cortex area but with the RGCs pattern around the injured corpus callosum area. The BLBP positive cells also expressed CXCR4 and SOX2. Altogether, CXCL12 promotes the proliferation of neural precursor cells after TBI by combing to its receptor, CXCR4. The proliferating neural precursor cells presents radial glial cell like cells. The RGCs-like cells can differentiate into immature neurons and promote the migration of immature neurons.
为了研究 CXCL12 对创伤性脑损伤（TBI）后放射状胶质样细胞再生的影响。我们将 48 只大鼠随机分为 4 组：（1）假手术组，大鼠仅行颅骨切开术；（2）对照组，TBI 后向同侧皮质内注射生理盐水；（3）CXCL12 组，注射 CXCL12；（4）CXCL12+AMD3100 组，注射 CXCL12 和 AMD3100 的混合物。TBI 后 7 天，对脑组织进行 BrdU/Nestin、BLBP/Nestin、BLBP/Vimentin、BLBP/SOX2、BLBP/CXCR4、BLBP/DCX 的免疫荧光双标染色，Western Blot 检测 Nestin、BLBP、Vimentin 水平。与对照组相比，CXCL12 处理显著增加了损伤皮质和胼胝体区域周围 BrdU/Nestin、BLBP/Nestin 和 BLBP/Vimentin 染色的细胞数量。与 CXCL12 处理和对照组相比，CXCL12+AMD3100 处理显著减少了这些细胞的数量。Nestin、BLBP 和 Vimentin 的蛋白水平与免疫荧光染色具有相同的变化趋势。BLBP/Vimentin 阳性细胞在损伤皮质区域呈星形胶质细胞形态，但在损伤胼胝体区域呈 RGC 形态。BLBP 阳性细胞还表达 CXCR4 和 SOX2。总之，CXCL12 通过与其受体 CXCR4 结合，促进 TBI 后神经前体细胞的增殖。增殖的神经前体细胞呈现放射状胶质样细胞。RGC 样细胞可分化为未成熟神经元，并促进未成熟神经元的迁移。
