School of Environment Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Lab of Indoor Air Environment Quality Control, Tianjin 300072, China.
School of Environment Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Lab of Indoor Air Environment Quality Control, Tianjin 300072, China.
Sci Total Environ. 2019 Dec 1;694:133750. doi: 10.1016/j.scitotenv.2019.133750. Epub 2019 Aug 2.
Antibiotic resistance genes (ARGs), as an emerging environmental pollutant, have received widespread attention. There are many studies on ARGs in water and soil. However, there are few studies on airborne ARGs. We used qPCR to detect 19 ARG subtypes in six atmospheric environments. Among the different atmospheric environments including wastewater treatment plant (fine screens and sludge thickener), bathroom, laboratory, hospital and outdoor, the highest total concentration of ARGs is detected in the haze outdoor (9 × 10 copies/m), while the lowest is in the bathroom atmosphere (4.2 × 10 copies/m). Furthermore, β-lactam ARGs are found to be the dominant ARGs in these 6 atmospheric environments. Due to the large number and widespread use of antibiotics, the airborne ARGs in hospital have the highest diversity and equitability. The β-lactam ARGs are significantly positively correlated with sulfonamides ARGs, indicating the mechanism of co-resistance since these two ARGs may be on the same genetic elements and thus simultaneously exhibit both resistances. The network analysis provides potential host information between the airborne ARGs and the coexisted microbial taxa. Sphingomonas and Bradyhizoblum strains presumably host for tetracycline and β-lactam ARGs, respectively. The ADD of bla was 7.8 × 10 copies/d/kg, more than the ADD (7.6 × 10 copies/d/kg) of bla by drinking water. We can't ignore ARGs in the atmospheric environments.
抗生素耐药基因（ARGs）作为一种新兴的环境污染物，受到了广泛关注。已有许多关于水和土壤中 ARGs 的研究，但关于空气中 ARGs 的研究较少。我们使用 qPCR 检测了六种大气环境中的 19 种 ARG 亚型。在包括污水处理厂（细格栅和污泥浓缩池）、浴室、实验室、医院和户外在内的不同大气环境中，霾户外空气中的 ARG 总浓度最高（9×10 拷贝/m），而浴室空气中的 ARG 总浓度最低（4.2×10 拷贝/m）。此外，β-内酰胺类 ARGs 是这 6 种大气环境中的主要 ARGs。由于抗生素的大量使用，医院空气中的 ARGs 具有最高的多样性和均匀度。β-内酰胺类 ARGs 与磺胺类 ARGs 呈显著正相关，表明存在共耐药机制，因为这两种 ARGs 可能位于同一遗传元件上，因此同时表现出两种耐药性。网络分析提供了空气中 ARGs 与共存微生物类群之间的潜在宿主信息。鞘氨醇单胞菌和布氏杆菌菌株可能分别携带四环素和β-内酰胺类 ARGs。bla 的 ADD 为 7.8×10 拷贝/d/kg，高于饮用水中 bla 的 ADD（7.6×10 拷贝/d/kg）。我们不能忽视大气环境中的 ARGs。
