School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
Institute of Dental Research, Centre for Oral Health, University of Sydney, Sydney, Australia.
Biochem Biophys Res Commun. 2019 Oct 8;518(1):44-49. doi: 10.1016/j.bbrc.2019.08.006. Epub 2019 Aug 7.
Campylobacter concisus is an emerging bacterial pathogen that may play a role in the development of inflammatory bowel disease and oral inflammatory conditions such as periodontal disease. To elucidate the role and pathogenic mechanisms of C. concisus in contributing to oral inflammation, this study examined the production of IL-1 family proinflammatory cytokines IL-18 and IL-1β in oral epithelial cells induced by C. concisus strains using enzyme-linked immunosorbent assay (ELISA), Western-blot and quantitative real-time PCR. C. concisus increased the mRNA levels of IL-18 and IL-1β in oral epithelial cells. Furthermore, a large amount of IL-18 in the supernatants of oral epithelial cells infected with C. concisus strains was detected by ELISA, and various experiments demonstrated that this positive signal was derived from C. concisus bacterium. The findings that C. concisus upregulated IL-18 and IL-1β in oral epithelial cells from this study support a role of C. concisus in oral inflammatory diseases. Furthermore, the finding that C. concisus released a molecule that was strongly cross-reactive to anti-human IL-18 monoclonal antibodies suggests that in future studies examining cytokines induced by bacterial microbes, a bacterium control should be included.
短小弯曲杆菌是一种新兴的细菌病原体，它可能在炎症性肠病和口腔炎症性疾病（如牙周病）的发展中发挥作用。为了阐明短小弯曲杆菌在导致口腔炎症中的作用和致病机制，本研究通过酶联免疫吸附试验（ELISA）、Western blot 和实时定量 PCR 检测了短小弯曲杆菌菌株诱导口腔上皮细胞产生的白细胞介素 1 家族促炎细胞因子白细胞介素 18 和白细胞介素 1β。短小弯曲杆菌增加了口腔上皮细胞中白细胞介素 18 和白细胞介素 1β 的 mRNA 水平。此外，通过 ELISA 检测到感染短小弯曲杆菌菌株的口腔上皮细胞上清液中大量的白细胞介素 18，并且各种实验证明该阳性信号源自短小弯曲杆菌。本研究发现短小弯曲杆菌在上皮细胞中上调了白细胞介素 18 和白细胞介素 1β，这支持短小弯曲杆菌在口腔炎症性疾病中的作用。此外，发现短小弯曲杆菌释放了一种与抗人白细胞介素 18 单克隆抗体强烈交叉反应的分子，这表明在未来研究细菌微生物诱导的细胞因子时，应包括细菌对照。
