Lee Seul A, Liu Fang, Yun Doo Young, Riordan Stephen M, Tay Alfred Chin Yen, Liu Lu, Lee Cheok Soon, Zhang Li
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
Gastrointestinal and Liver Unit,Prince of Wales Hospital, University of New South Wales, Sydney, Australia.
J Oral Microbiol. 2021 Sep 14;13(1):1978732. doi: 10.1080/20002297.2021.1978732. eCollection 2021.
is an oral bacterium that is associated with inflammatory bowel disease (IBD) and Barrett's esophagus (BE). Programmed cell death ligand-1 (PD-L1) is an immune checkpoint protein that is used by tumor cells for immune evasion and has increased expression in patients with IBD and BE. We examined whether upregulates PD-L1 expression in intestinal and esophageal epithelial cells. Human intestinal epithelial HT-29 cells and esophageal epithelial FLO-1 cells with and without interferon (IFN)-γ sensitization were incubated with strains. The level of PD-L1 mRNA was quantified using quantitative real-time PCR. Cytokines were measured using Enzyme-Linked Immunosorbent Assay (ELISA). Apoptosis of HT-29 and FLO-1 cells were measured using caspase 3/7 assay. We found that intestinal epithelial cells with IFN-γ sensitization incubated with significantly upregulated PD-L1 expression and significantly increased the production of interleukin (IL)-8. Whereas, PD-L1 expression was significantly inhibited in IFN-γ sensitized FLO-1 cells incubated with strains. Furthermore, FLO-1 cells with and without IFN-γ sensitization incubated with strains both had significantly higher levels of cell death. has the potential to cause damage to both intestinal and esophageal epithelial cells, however, with different pathogenic effects.
是一种与炎症性肠病（IBD）和巴雷特食管（BE）相关的口腔细菌。程序性细胞死亡配体-1（PD-L1）是一种免疫检查点蛋白，肿瘤细胞利用它进行免疫逃逸，并且在IBD和BE患者中表达增加。我们研究了[细菌名称未给出]是否会上调肠道和食管上皮细胞中PD-L1的表达。将有或没有干扰素（IFN）-γ致敏的人肠道上皮HT-29细胞和食管上皮FLO-1细胞与[细菌名称未给出]菌株一起孵育。使用定量实时PCR对PD-L1 mRNA水平进行定量。使用酶联免疫吸附测定（ELISA）测量细胞因子。使用半胱天冬酶3/7测定法测量HT-29和FLO-1细胞的凋亡。我们发现，与[细菌名称未给出]一起孵育的经IFN-γ致敏的肠道上皮细胞显著上调了PD-L1表达，并显著增加了白细胞介素（IL）-8的产生。然而，在与[细菌名称未给出]菌株一起孵育的经IFN-γ致敏的FLO-1细胞中，PD-L1表达受到显著抑制。此外，与[细菌名称未给出]菌株一起孵育的有或没有IFN-γ致敏的FLO-1细胞的细胞死亡水平均显著更高。[细菌名称未给出]有可能对肠道和食管上皮细胞都造成损害，然而，具有不同的致病作用。
