Department of Microbial and Molecular Systems (M2S), KULeuven, 3001, Heverlee, Belgium.
Gembloux Agro-Bio tech, TERRA Research and Teaching Center, Microbial Processes and Interactions, University of Liège, Avenue de la Faculté 2B, 5030, Gembloux, Belgium.
Bioprocess Biosyst Eng. 2019 Dec;42(12):1935-1946. doi: 10.1007/s00449-019-02187-6. Epub 2019 Aug 10.
Relative to the amount of knowledge concerning bacterial biofilms, little is known about the impact of physico-chemical properties of support material on fungal biofilm adhesion and physiology. In the field of industrial fermentation, large-scale production of low-cost fungal secondary product is a challenging area of research. In the present work, the effect of physico-chemical surface properties of five different materials (Teflon, glass, Viton™ rubber, silicon rubber, and stainless steel) on the production of class II hydrophobins (HFBI and HFBII) from Trichoderma reesei (HFB2a-2) and Trichoderma harzianum) was evaluated. Two culture systems (shake flask and drip flow reactor (DFR)) were used in this study to promote biomass growth and the production of hydrophobins. Furthermore, the effect of physico-chemical surface properties (hydrophobicity, surface energy) and surface texture (roughness) of support material on the initial colonization and attachment of the fungal biofilm was evaluated. Maximum biofilm productivity was obtained using Viton™ rubber for T. reesei and Viton™ rubber and stainless steel as support materials for T. harzianum. Scanning electron microscope (SEM) revealed that fungal biofilm adhesion was higher on the rough hydrophobic Viton rubber surface as compared to the smooth hydrophobic Teflon surface. Initial colonization initiated because of surface irregularities and holes in the material as hyphal filaments. Moreover, compared to traditional submerged fermentation, a significant increase in biofilm productivity for both strains (T. reesei, T. harzianum) in all five materials was obtained.
相对于细菌生物膜的知识量，对于支持材料的物理化学性质对真菌生物膜附着和生理学的影响知之甚少。在工业发酵领域，大规模生产低成本真菌次级产物是一个具有挑战性的研究领域。在本工作中，研究了五种不同材料（特氟龙、玻璃、Viton™橡胶、硅橡胶和不锈钢）的物理化学表面性质对里氏木霉（HFB2a-2）和哈茨木霉（HFB2a-2）产生 II 类疏水蛋白（HFBI 和 HFBII）的影响。Trichoderma harzianum）。本研究使用了两种培养系统（摇瓶和滴流反应器（DFR））来促进生物量生长和疏水蛋白的生产。此外，还评估了支撑材料的物理化学表面性质（疏水性、表面能）和表面纹理（粗糙度）对真菌生物膜初始定殖和附着的影响。对于里氏木霉，Viton™橡胶获得了最大的生物膜生产力，而对于哈茨木霉，Viton™橡胶和不锈钢是支持材料。扫描电子显微镜（SEM）显示，与光滑疏水的特氟龙表面相比，真菌生物膜在粗糙疏水的 Viton 橡胶表面上的附着更高。由于材料中的表面不规则和孔，初始定殖是由菌丝丝发起的。此外，与传统的浸没发酵相比，两种菌株（里氏木霉、哈茨木霉）在所有五种材料中的生物膜生产力都显著提高。
