Dental Materials Laboratory, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Oral and Maxillofacial Surgery Unit, School of Dentistry, Universidade Federal do Rio Grande do Sul, Brazil.
Dent Mater. 2019 Oct;35(10):1490-1497. doi: 10.1016/j.dental.2019.07.012. Epub 2019 Aug 9.
The aim of this study is to investigate bone healing ability of niobium-containing bioactive glasses in rat femur model with quantitative and qualitative measurements through x-ray computed microtomography.
Niobium-containing bioactive powders and scaffolds were produced by sol-gel route (BAGNb). Glasses without niobium addition were produced as well (BAG). Five groups were used: BAGNb powders, BAG powders, BAGNb scaffolds, BAG scaffolds and, as a control group, autogenous bone was used. Materials were implanted in the femur of male rats (Wistar Lineage n=10) and the healing was observed after 15, 30 and 60 days. After the post-operative times, samples were scanned by X-ray microcomputed tomography where morphometric measurements and the mineral density were assessed in image software.
No postoperative complications were observed after surgery. BAGNb glasses presented higher mineral deposition, which was observed in the relative volume of bone and the mineral density when compared BAG groups. In these parameters, no statistical difference was found between BAGNb and autogenous bone. The BAGNb powders presented a higher amount of mineralized tissue when compared to BAGNb scaffolds. The analysis of trabecular structure showed lower trabecular formation in synthetic materials when compared to autogenous bone.
Niobium-containing bioactive glasses promoted bone formation comparable to that of the autogenous bone without compromising the quality of the formed bone.
本研究旨在通过 X 射线计算机断层扫描（X-ray computed microtomography）对含铌生物活性玻璃在大鼠股骨模型中的骨愈合能力进行定量和定性测量。
采用溶胶-凝胶法（BAGNb）制备含铌生物活性粉末和支架，同时制备不含铌的玻璃（BAG）。共设 5 组：BAGNb 粉末、BAG 粉末、BAGNb 支架、BAG 支架和作为对照组的自体骨。将材料植入雄性大鼠（Wistar 系 n=10）的股骨中，分别在术后 15、30 和 60 天观察愈合情况。术后，采用 X 射线微计算机断层扫描对样品进行扫描，在图像软件中评估形态计量学测量和骨密度。
手术后无术后并发症。与 BAG 组相比，BAGNb 玻璃表现出更高的矿化沉积，表现为相对骨体积和骨密度的增加。在这些参数中，BAGNb 与自体骨之间没有统计学差异。与 BAGNb 支架相比，BAGNb 粉末具有更高的矿化组织量。小梁结构分析显示，与自体骨相比，合成材料的小梁形成较少。
含铌生物活性玻璃促进骨形成的能力可与自体骨相媲美，而不会影响形成骨的质量。
