Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary; Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary.
Department of Pathology, Medical School, University of Pécs, Pécs, Hungary.
Infect Genet Evol. 2019 Nov;75:103995. doi: 10.1016/j.meegid.2019.103995. Epub 2019 Aug 9.
Retroviruses (family Retroviridae) are important agents of humans and animals. This study reports the detection and complete genome characterization of a novel endogenous retrovirus from the black Syrian hamster (Mesocricetus auratus) with a squamous cell skin tumor. The proviral genome, tentatively named black Syrian hamster retrovirus (BSHRV/2013/HUN, MK304634), was 8784 nucleotide in length with typical full-length betaretrovirus genome organization of 5'LTR-gag-pro-pol-env-3'LTR and with a characteristic mouse mammary tumor virus-like (MMTV) betaretrovirus dUTPase domain but without a sag gene. The BSHRV gag (534aa), pro/pol (~1099aa) and env (672aa) proteins had 56%/63%/50% aa identity to the corresponding proteins of MMTV (AF228552). The proviral DNA is detectable in tumor as well as in tumor-free cells by conventional PCR and qPCR but only visible in the tumor cells by in situ hybridization. Low level retroviral RNA expression was found only in the DNase-treated RNA tumor samples using RT/nested PCR. BSHRV/2013/HUN-like betaretrovirus DNA was also identified from a faecal and tissue samples from 1 of the further 3 tested individuals by nested-PCR and qPCR. Further research is needed to investigate the distribution, activity and etiological role of this novel MMTV-like betaretrovirus species in hamster.
逆转录病毒（Retroviridae 科）是人类和动物的重要病原体。本研究报道了从患有鳞状细胞皮肤肿瘤的黑叙利亚仓鼠（Mesocricetus auratus）中检测到并完全鉴定其基因组特征的一种新型内源性逆转录病毒。该前病毒基因组，暂定名为黑叙利亚仓鼠逆转录病毒（BSHRV/2013/HUN，MK304634），长度为 8784 个核苷酸，具有典型的全长β逆转录病毒基因组结构 5'LTR-gag-pro-pol-env-3'LTR，并具有特征性的鼠乳腺肿瘤病毒样（MMTV）β逆转录病毒 dUTP 酶结构域，但没有 sag 基因。BSHRV gag（534aa）、pro/pol（~1099aa）和 env（672aa）蛋白与 MMTV（AF228552）相应蛋白的氨基酸同一性分别为 56%/63%/50%。通过常规 PCR 和 qPCR 可在肿瘤以及肿瘤无细胞中检测到前病毒 DNA，但通过原位杂交仅可在肿瘤细胞中观察到。仅在用 DNAse 处理的 RNA 肿瘤样本中使用 RT/嵌套 PCR 才可检测到低水平的逆转录病毒 RNA 表达。通过嵌套-PCR 和 qPCR 还从另外 3 只检测个体中的 1 只的粪便和组织样本中鉴定出了 BSHRV/2013/HUN 样β逆转录病毒 DNA。需要进一步研究来调查这种新型 MMTV 样β逆转录病毒在仓鼠中的分布、活性和病因学作用。
