College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China; The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-Based Materials, Anhui Key Laboratory of Chemo-Biosensing, PR China.
College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China; The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-Based Materials, Anhui Key Laboratory of Chemo-Biosensing, PR China.
Bioelectrochemistry. 2019 Dec;130:107344. doi: 10.1016/j.bioelechem.2019.107344. Epub 2019 Aug 2.
In this work, a novel electrochemical sensing platform was designed and fabricated by the modification of boronic acid functionalized carbon nanodots (B-CNDs) and poly(thionine) (pTHI) on an electrode surface. B-CNDs can not only accelerate electron transfer but also covalently interact with cis-diol groups of dihydronicotinamide adenine dinucleotide (NADH) through functionalized boronic acid groups. Meanwhile, pTHI served as an inner reference element to provide a built-in correction, which enabled the sensor to detect NADH with high accuracy and reliability based on a ratiometric signal (∆I/∆I). The electrochemical experimental results demonstrated that the ratiometric strategy-based sensor possessed good selectivity and high sensitivity. A linear range of 5.0 × 10 - 2.0 × 10 mol/L for NADH detection was obtained with a limit of detection of 1.5 × 10 mol/L. The sensor has been applied to analyze NADH in human serum samples with satisfactory results. The simple and effective ratiometric strategy reported here can be further used to prepare electrochemical sensors for selective, sensitive, and reliable detection of other cis-diol compounds.
在这项工作中，通过在电极表面修饰硼酸功能化碳点（B-CNDs）和聚噻吩（pTHI），设计并制造了一种新型电化学传感平台。B-CNDs 不仅可以加速电子转移，而且可以通过功能化硼酸基团与二氢烟酰胺腺嘌呤二核苷酸（NADH）的顺二醇基团发生共价相互作用。同时，pTHI 作为内参元素提供内置校正，使传感器能够基于比率信号（∆I/∆I）以高准确度和可靠性检测 NADH。电化学实验结果表明，基于比率策略的传感器具有良好的选择性和高灵敏度。对于 NADH 检测，获得了 5.0×10 - 2.0×10 mol/L 的线性范围，检测限为 1.5×10 mol/L。该传感器已成功应用于分析人血清样品中的 NADH，获得了令人满意的结果。这里报道的简单有效的比率策略可以进一步用于制备电化学传感器，用于选择性、灵敏和可靠地检测其他顺二醇化合物。
