Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.
Biosens Bioelectron. 2019 Oct 1;142:111564. doi: 10.1016/j.bios.2019.111564. Epub 2019 Aug 2.
Solid-state nanochannels have great potentials in the vibrant field of photoelectrochemical (PEC) bioanalysis. This work herein demonstrates the innovative use of DNA-decorated nanoporous anodic alumina (NAA) nanochannels for sensitive PEC bioanalysis of telomerase (TE) activity. Specifically, telomerase primer sequences (TS) were initially immobilized within the NAA nanochannels and then extended by TE in the presence of deoxyribonucleoside triphosphates (dNTPs). The as formed single-strand DNA was then directed to hybrid with many partially matched single-strand assisting DNA (aDNA), leading to the formation of multiple DNAzymes by the unmatched parts and the subsequent DNAzyme-stimulated biocatalytic precipitation (BCP) within the nanochannels. Because the inhibited signals of the photoelectrode could be correlated with TE-enabled TS extension, an innovative nanochannels PEC bioanalysis could be realized for probing TE activity. This work features the ingenious use of DNA-associated nanochannels for PEC bioanalysis of TE activity. Given the versatile functions of DNA molecules, the extension of this strategy easily allows for addressing numerous other targets of interest. Also, we envision this work could inspire more interest for the further development of nanochannels PEC bioanalysis.
固态纳米通道在光电化学（PEC）生物分析这一充满活力的领域具有巨大的潜力。本工作创新性地利用 DNA 修饰的多孔阳极氧化铝（NAA）纳米通道，用于端粒酶（TE）活性的灵敏 PEC 生物分析。具体而言，TE 酶引物序列（TS）首先固定在 NAA 纳米通道内，然后在脱氧核糖核苷三磷酸（dNTPs）存在下通过 TE 延伸。形成的单链 DNA 随后与许多部分匹配的单链辅助 DNA（aDNA）杂交，导致不匹配部分形成多个 DNA 酶，并随后在纳米通道内发生 DNA 酶刺激的生物催化沉淀（BCP）。由于光电极的抑制信号可以与 TE 介导的 TS 延伸相关联，因此可以实现用于探测 TE 活性的创新纳米通道 PEC 生物分析。本工作的特点是巧妙地利用 DNA 相关纳米通道进行 TE 活性的 PEC 生物分析。鉴于 DNA 分子的多功能性，该策略的扩展可以轻松地针对其他许多感兴趣的目标。此外，我们设想这项工作可以激发更多人对纳米通道 PEC 生物分析的进一步发展的兴趣。
