State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Department of Materials Science and Engineering, Stanford University , Stanford, California 94305, United States.
Anal Chem. 2018 Feb 6;90(3):2341-2347. doi: 10.1021/acs.analchem.7b04862. Epub 2018 Jan 5.
Nanochannels have brought new opportunities for biosensor development. Herein, we present the novel concept of a nanochannels photoelectrochemical (PEC) biosensor based on the integration of a unique CuO-nanopyramid-islands (NPIs) photocathode, an anodic aluminum oxide (AAO) membrane, and alkaline phosphatase (ALP) catalytic chemistry. The CuO-NPIs photocathode possesses good performance, and further assembly with AAO yields a designed architecture composed of vertically aligned, highly ordered nanoarrays on top of the CuO-NPIs film. After biocatalytic precipitation (BCP) was stimulated within the channels, the biosensor was used for the successful detection of ALP activity. This study has not only provided a novel paradigm for an unconventional nanochannels PEC biosensor, which can be used for general bioanalytical purposes, but also indicated that the new concept of nanochannel-semiconductor heterostructures is a step toward innovative biomedical applications.
纳米通道为生物传感器的发展带来了新的机遇。在此，我们提出了一种基于独特的氧化铜纳米金字塔岛（CuO-NPIs）光电阴极、氧化铝阳极（AAO）膜和碱性磷酸酶（ALP）催化化学的纳米通道光电化学（PEC）生物传感器的新概念。CuO-NPIs 光电阴极具有良好的性能，进一步与 AAO 组装可得到由垂直排列、高度有序的纳米阵列组成的设计结构，位于 CuO-NPIs 薄膜之上。在通道内刺激生物催化沉淀（BCP）后，该生物传感器成功用于检测 ALP 活性。这项研究不仅为一种可用于一般生物分析目的的非常规纳米通道 PEC 生物传感器提供了新的范例，还表明了纳米通道-半导体异质结构的新概念是迈向创新的生物医学应用的一步。
