Department of Cardiovascular Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, P.R. China.
Cardiovascular Department, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, Zhejiang, China.
Sci Rep. 2019 Aug 12;9(1):11599. doi: 10.1038/s41598-019-47853-7.
Neutrophil extracellular traps (NETs) which have a potential role in noninfectious diseases, may play an important role in patients with acute coronary syndrome. The goal of this study was to investigate the association of NETs and in-hospital major adverse cardiac events among patients with ST-segment elevation myocardial infarction (STEMI). Using immunofluorescence staining, ELISA, and fluorescent enzyme standard instrument, we assessed NETs and NETs-related factors. Multivariate analyses were performed after univariate analyses to investigate which variables were independently associated with major adverse cardiac events. Compared with peripheral arteries, we observed neutrophils obtained from infarct-related artery (IRA) releasing NETs. The dsDNA levels, NET-specific marker myeloperoxidase/deoxyribonucleic acid (MPO/DNA) complexes and NETs-related factor tissue factor were significantly higher in coronary plasma samples. Multivariate analysis that white cell counts and coronary dsDNA were independently associated with in-hospital major adverse cardiac events. ROC curve for coronary dsDNA showed sensitivity of 78.0% and specificity of 53% for the cut-off value of 0.39ug/ml. Conclusion, these results provide evidences indicating NETs were associated with STIM, and occurrence of adverse cardiac events.
中性粒细胞胞外诱捕网（NETs）在非传染性疾病中具有潜在作用，可能在急性冠状动脉综合征患者中发挥重要作用。本研究旨在探讨 NETs 与 ST 段抬高型心肌梗死（STEMI）患者住院期间主要不良心脏事件之间的关系。通过免疫荧光染色、ELISA 和荧光酶标仪，我们评估了 NETs 和 NETs 相关因素。对单变量分析后进行多变量分析，以调查哪些变量与主要不良心脏事件独立相关。与外周动脉相比，我们观察到来自梗死相关动脉（IRA）的中性粒细胞释放 NETs。在冠状血浆样本中，dsDNA 水平、NET 特异性标志物髓过氧化物酶/脱氧核糖核酸（MPO/DNA）复合物和 NETs 相关因子组织因子明显更高。多变量分析显示，白细胞计数和冠状动脉 dsDNA 与住院期间主要不良心脏事件独立相关。冠状动脉 dsDNA 的 ROC 曲线显示，截断值为 0.39ug/ml 时，其对不良心脏事件的敏感性为 78.0%，特异性为 53%。结论，这些结果提供了证据，表明 NETs 与 STEMI 和不良心脏事件的发生有关。
