Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, 01003, USA.
Sci Rep. 2019 Aug 12;9(1):11648. doi: 10.1038/s41598-019-48206-0.
The dissipation patterns of cyantraniliprole and its metabolite IN-J9Z38 were investigated using proso millet (Panicum miliaceum) under open-field conditions to establish a pre-harvest interval. A simple and sensitive analytical method was developed for analyzing residues using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for multiple reaction monitoring of target compounds. The analytical method was validated in terms of the instrumental limit of quantitation, method limit of quantitation, linearity, accuracy, and precision. The method was successfully applied to the analysis of cyantraniliprole and IN-J9Z38 residues in the field samples of four plots, which were treated twice with an oil dispersion formulation, according to the date of pesticide treatment before harvest. In the case of cyantraniliprole in grain and straw, there was a 91.1 and 89.1% decrease, respectively, from the initial residues (14-7 days) to the final plot (40-30 days before harvest). However, IN-J9Z38 gradually increased over time, indicating that cyantraniliprole transformed into IN-J9Z38 during cultivation. The biological half-lives of total cyantraniliprole were 11.3 and 9.4 days for grain and straw, respectively. The results obtained in this study will inform regulation and management of pesticide use for the minor crop proso millet.
在田间条件下，用谷子（ Panicum miliaceum ）研究了虫螨腈及其代谢物 IN-J9Z38 的消解动态，以确定其收获前间隔期。采用超高效液相色谱-串联质谱法（ UHPLC-MS/MS ），建立了一种用于分析残留的简单、灵敏的分析方法，采用多反应监测对目标化合物进行定量分析。从仪器定量限、方法定量限、线性、准确度和精密度等方面对分析方法进行了验证。该方法成功应用于四个试验区两次用油分散制剂处理的田间样品中，根据收获前农药处理日期，对谷物和秸秆中的虫螨腈和 IN-J9Z38 残留进行了分析。在谷物和秸秆中，虫螨腈的初始残留量（ 14-7 天）分别减少了 91.1%和 89.1%，最终残留量（收获前 40-30 天）。然而， IN-J9Z38 随着时间的推移逐渐增加，表明虫螨腈在种植过程中转化为 IN-J9Z38 。总虫螨腈在谷物和秸秆中的生物半衰期分别为 11.3 天和 9.4 天。本研究结果将为小宗作物谷子的农药使用管理提供依据。
