Bose Susmita, Banerjee Dishary, Shivaram Anish, Tarafder Solaiman, Bandyopadhyay Amit
W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington, 99164-2920, USA.
Mater Des. 2018 Aug 5;151:102-112. doi: 10.1016/j.matdes.2018.04.049. Epub 2018 Apr 18.
This study aims to improve the interfacial bonding between the osseous host tissue and the implant surface through the application of doped calcium phosphate (CaP) coating on 3D printed porous titanium. Porous titanium (Ti) cylinders with 25% volume porosity were fabricated using Laser Engineered Net Shaping (LENS™), a commercial 3D Printing technique. The surface of these 3D printed cylinders was modified by growing TiO nanotubes first, followed by a coating of with Sr and Si doped bioactive CaP ceramic in simulated body fluid (SBF). Doped CaP coated implants were hypothesized to show enhanced early stage bone tissue integration. Biological properties of these implants were investigated using a rat distal femur model after 4 and 10 weeks. CaP coated porous Ti implants have enhanced tissue ingrowth as was evident from the CT scan analysis, push out test results, and the histological analysis compared to porous implants with or without surface modification via titania nanotubes. Increased osteoid-like new bone formation and accelerated mineralization was revealed inside the CaP coated porous implants. It is envisioned that such an approach of adding a bioactive doped CaP layer on porous Ti surface can reduce healing time by enhancing early stage osseointegration .
本研究旨在通过在3D打印的多孔钛上应用掺杂磷酸钙（CaP）涂层，改善骨宿主组织与植入物表面之间的界面结合。使用商业3D打印技术激光工程化净成形（LENS™）制造了孔隙率为25%的多孔钛（Ti）圆柱体。首先通过生长TiO纳米管对这些3D打印圆柱体的表面进行改性，然后在模拟体液（SBF）中用Sr和Si掺杂的生物活性CaP陶瓷进行涂层。假设掺杂CaP涂层的植入物在早期骨组织整合方面表现出增强效果。在4周和10周后，使用大鼠股骨远端模型研究了这些植入物的生物学特性。与经过或未经过二氧化钛纳米管表面改性的多孔植入物相比，CaP涂层多孔Ti植入物的组织向内生长增强，这从CT扫描分析、推出试验结果和组织学分析中可以明显看出。在CaP涂层多孔植入物内部发现类骨质新骨形成增加且矿化加速。可以设想，在多孔Ti表面添加生物活性掺杂CaP层的这种方法可以通过增强早期骨整合来减少愈合时间。
