Olson D M, Smieja Z
Department of Paediatrics, University of Western Ontario, London, Ontario, Canada.
Am J Obstet Gynecol. 1988 Oct;159(4):995-1001. doi: 10.1016/s0002-9378(88)80187-x.
There were no differences in the rate or amount of (1-14C)-labeled arachidonic acid incorporated into triacylglycerides, diacylglycerides, or any phospholipid species of freshly dispersed term human amnion cells obtained before or after labor. Both phosphatidylcholine and phosphatidylethanolamine incorporated 14C-arachidonic acid in proportion to their molar percent of total amnion phospholipids, but phosphatidylinositol incorporated three times as much 14C-arachidonic acid, suggesting either a rapid turnover in this specific phospholipid pool or a greater specificity for the transfer of arachidonoyl-coenzyme A to lysophosphatidylinositol. No or little competition of 14C-arachidonic acid incorporation into triacylglycerides or phospholipids occurred with palmitic acid, linoleic acid, or gamma-linolenic acid. However, dihomo-gamma-linolenic acid, eicosapentaenoic acid, and unlabeled arachidonic acid were effective inhibitors. We conclude that the term amnion has high acyl transferase activity, that no change in the basal activity of this enzyme occurs with the onset of labor, and that a specific acyl transferase exists for 20-carbon polyunsaturated fatty acids.
分娩前或分娩后获取的新鲜分离的足月人羊膜细胞中，（1-14C）标记的花生四烯酸掺入甘油三酯、甘油二酯或任何磷脂种类的速率或量没有差异。磷脂酰胆碱和磷脂酰乙醇胺按其在羊膜总磷脂中所占摩尔百分比的比例掺入14C-花生四烯酸，但磷脂酰肌醇掺入的14C-花生四烯酸是前者的三倍，这表明该特定磷脂池要么周转迅速，要么花生四烯酰辅酶A向溶血磷脂酰肌醇的转移具有更高的特异性。棕榈酸、亚油酸或γ-亚麻酸对14C-花生四烯酸掺入甘油三酯或磷脂没有竞争作用，或竞争作用很小。然而，二高-γ-亚麻酸、二十碳五烯酸和未标记的花生四烯酸是有效的抑制剂。我们得出结论，足月羊膜具有高酰基转移酶活性，分娩开始时该酶的基础活性没有变化，并且存在针对20碳多不饱和脂肪酸的特异性酰基转移酶。
