Strugnell R A, Kent T, Handley C J, Faine S
Department of Microbiology, Monash Medical School, Alfred Hospital, Prahran, Victoria, Australia.
Am J Pathol. 1988 Oct;133(1):110-7.
The relationship between Treponema pallidum infection and the synthesis of proteoglycans by organ cultures of rabbit testes was investigated. Two proteoglycan fractions, large (CL-6B Kav 0.05) and small (Kav 0.25), that were not synthesized at detectable levels by cultures from rabbits infected with T. pallidum for 0 or 5 days, were produced by cultures from 10-, 15- and 20-day infected rabbits. The small proteoglycan appeared to be synthesized first because greater amounts of this fraction were detected in extracts of cultures from 10-day infected animals. The large proteoglycan fraction may have been induced directly by T. pallidum because increased synthesis correlated with maximal treponemal numbers, 15 days after infection. In contrast, the induction of the smaller proteoglycan did not appear to be directly related to numbers of elutable organisms. The proteoglycans synthesized between 10 and 20 days after infection were analyzed for glycosaminoglycan (GAG) size and composition. The size of GAGs beta-eliminated from the proteoglycans generally increased over the 10-20-day infection period. Whereas the composition of the small proteoglycan fraction was largely unchanged during the 10-20-day period (45% chondroitin sulphate (CS), 55% dermatan sulphate (DS)), the amount of DS in the high molecular weight proteoglycan fraction decreased from 50-20% during this period (CS 50-80%). Autoradiography studies revealed that increased proteoglycan synthesis in T. pallidum-infected testes was localized to cells lining the seminiferous tubules and to fibroblasts infiltrating peritubular spaces.
研究了梅毒螺旋体感染与兔睾丸器官培养物中蛋白聚糖合成之间的关系。两种蛋白聚糖组分，大分子（CL - 6B Kav 0.05）和小分子（Kav 0.25），在感染梅毒螺旋体0天或5天的兔培养物中未检测到有合成，但在感染10天、15天和20天的兔培养物中产生。小分子蛋白聚糖似乎先合成，因为在感染10天动物的培养物提取物中检测到更多的该组分。大分子蛋白聚糖组分可能是由梅毒螺旋体直接诱导的，因为合成增加与感染后15天的最大螺旋体数量相关。相比之下，较小蛋白聚糖的诱导似乎与可洗脱生物体的数量没有直接关系。分析了感染后10至20天合成的蛋白聚糖的糖胺聚糖（GAG）大小和组成。从蛋白聚糖中β-消除的GAG大小在10至20天的感染期内通常会增加。在10至20天期间，小分子蛋白聚糖组分的组成基本不变（45%硫酸软骨素（CS），55%硫酸皮肤素（DS）），而高分子量蛋白聚糖组分中的DS量在此期间从50%降至20%（CS从50%升至80%）。放射自显影研究表明，梅毒螺旋体感染的睾丸中蛋白聚糖合成增加定位于生精小管内衬细胞和浸润肾小管周围间隙的成纤维细胞。
