Wong G H, Steiner B M, Graves S R
Br J Vener Dis. 1982 Jun;58(3):139-42. doi: 10.1136/sti.58.3.139.
Treponema pallidum extracted from infected rabbit testes under anaerobic conditions survived longer in vitro than those extracted under aerobic conditions. Anaerobically extracted treponemes were incubated anaerobically for 0, 12, 24, 36, or 48 hours and then exposed to microaerophilic conditions (3% oxygen) for further incubation. Treponemes transferred to microaerophilic conditions after 36 or 48 hours' anaerobic incubation maintained significantly greater viability compared with those kept under constant microaerophilic conditions, although there was no difference after 12 or 24 hours. T pallidum incubated under constant anaerobic conditions, however, usually maintained greater viability than those kept under constant microaerophilic conditions. These results suggest that T pallidum is sensitive to oxygen toxicity both during initial extraction from orchitic rabbit testes and subsequent incubation in vitro. In the latter case, it can be partially protected by a period of anaerobic incubation in vitro, before exposure to microaerophilic conditions.
在厌氧条件下从受感染兔睾丸中提取的梅毒螺旋体在体外存活时间比在需氧条件下提取的更长。将厌氧提取的螺旋体在厌氧条件下孵育0、12、24、36或48小时，然后暴露于微需氧条件（3%氧气）下进一步孵育。与在恒定微需氧条件下保存的螺旋体相比，厌氧孵育36或48小时后转移到微需氧条件下的螺旋体保持了显著更高的活力，尽管在12或24小时后没有差异。然而，在恒定厌氧条件下孵育的梅毒螺旋体通常比在恒定微需氧条件下保存的螺旋体保持更高的活力。这些结果表明，梅毒螺旋体在从睾丸炎兔睾丸中最初提取以及随后的体外孵育过程中对氧毒性敏感。在后一种情况下，在暴露于微需氧条件之前，通过一段时间的体外厌氧孵育可以对其起到部分保护作用。
