通过数量和亮度分析监测单个活细胞中的转录因子寡聚化

Monitoring Transcription Factor Oligomerization in Single Living Cells by Number and Brightness Analysis.

作者信息

Cammarota Eugenia, Mazza Davide

机构信息

Istituto Scientifico Ospedale San Raffaele, Centro di Imaging Sperimentale, Milan, Italy.

Fondazione CEN, European Center for Nanomedicine, Milan, Italy.

出版信息

Methods Mol Biol. 2019;2038:223-237. doi: 10.1007/978-1-4939-9674-2_15.

DOI:10.1007/978-1-4939-9674-2_15

PMID:31407288

Abstract

One key step in the activation of inducible transcription factors is their homooligomerization, which can be measured in individual living cells by a fluorescence microscopy technique called Number and Brightness analysis (N&B). In this chapter we describe how to acquire and analyze confocal microscopy time-series to provide information about transcription factor oligomerization in living cells using this technique.

摘要

诱导型转录因子激活过程中的一个关键步骤是它们的同型寡聚化，这可以通过一种称为数量与亮度分析（N&B）的荧光显微镜技术在单个活细胞中进行测量。在本章中，我们将描述如何获取和分析共聚焦显微镜时间序列，以利用该技术提供有关活细胞中转录因子寡聚化的信息。