Felipo V, Miñana M D, Grisolía S
Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Centro Asociado del CSIC Amadeo de Saboya, Spain.
Biochem Biophys Res Commun. 1988 Oct 14;156(1):506-10. doi: 10.1016/s0006-291x(88)80870-2.
Rats were fed standard (20% protein), protein-free or high protein (80%) diets for 15 days and then injected intraperitoneally with ammonium acetate (7 mmol/Kg). Survival was 6%, 75% and 100%, respectively, for rats fed standard, protein-free and high protein diets. After injection of 6 mmol/Kg of ammonium acetate, blood ammonia reached a peak (at ca. 2 mM) after 7, 25 and 30 min for rats fed high protein, protein-free and standard diets, respectively. The results presented indicate that protection in the high protein group is due to faster detoxication of ammonia via a more active urea cycle while the tolerance of the protein-free group to higher levels of ammonia remains to be clarified.
给大鼠分别喂食标准（20%蛋白质）、无蛋白或高蛋白（80%）饮食15天，然后腹腔注射醋酸铵（7 mmol/Kg）。喂食标准、无蛋白和高蛋白饮食的大鼠存活率分别为6%、75%和100%。注射6 mmol/Kg醋酸铵后，喂食高蛋白、无蛋白和标准饮食的大鼠血氨分别在7、25和30分钟后达到峰值（约2 mM）。给出的结果表明，高蛋白组的保护作用是由于通过更活跃的尿素循环更快地解毒氨，而无蛋白组对更高水平氨的耐受性仍有待阐明。
