MdWRKY46 通过激活水杨酸信号通路中 的表达增强苹果对 的抗性。
MdWRKY46-Enhanced Apple Resistance to by Activating the Expression of in the Salicylic Acid Signaling Pathway.
机构信息
State Key Laboratory of Crop Stress Biology for Arid Areas and Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China.
State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China.
出版信息
Mol Plant Microbe Interact. 2019 Oct;32(10):1391-1401. doi: 10.1094/MPMI-03-19-0089-R. Epub 2019 Aug 12.
Salicylic acid (SA) is closely related to disease resistance of plants. WRKY transcription factors have been linked to the growth and development of plants, especially under stress conditions. However, the regulatory mechanism of WRKY proteins involved in SA production and disease resistance in apple is not clear. In this study, responded to and enhanced resistance to . Electrophoretic mobility shift assays, yeast one-hybrid assays, and chromatin immunoprecipitation and quantitative PCR demonstrated that MdWRKY46 can directly bind to a W-box motif in the promoter of . Glucuronidase transactivation and luciferase analysis further showed that MdWRKY46 can activate the expression of . Finally, inoculation in transgenic apple calli and fruits revealed that MdWRKY46 improved resistance to by the transcriptional activation of . Viral vector-based transformation assays indicated that MdWRKY46 elevates SA content and transcription of SA-related genes, including , , and in an -dependent way. These findings provide new insights into how MdWRKY46 regulates plant resistance to through the SA signaling pathway.
水杨酸（SA）与植物的抗病性密切相关。WRKY 转录因子与植物的生长和发育有关，特别是在胁迫条件下。然而，参与苹果中 SA 产生和抗病性的 WRKY 蛋白的调控机制尚不清楚。在本研究中， 响应 并增强了对 的抗性。电泳迁移率变动分析、酵母单杂交分析、染色质免疫沉淀和定量 PCR 表明，MdWRKY46 可以直接结合 启动子中的 W-box 基序。β-葡萄糖醛酸酶转录激活和荧光素酶分析进一步表明，MdWRKY46 可以激活 的表达。最后，在转基因苹果愈伤组织和果实中的 接种表明，MdWRKY46 通过 的转录激活提高了对 的抗性。基于病毒载体的转化分析表明，MdWRKY46 以 依赖的方式提高 SA 含量和 SA 相关基因，包括 、 、 和 的转录。这些发现为 MdWRKY46 通过 SA 信号通路调节植物对 的抗性提供了新的见解。
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