Ovchinnikov A N, Deryugina A V
Lobachevsky State University of Nizhny Novgorod, Department of Physiology and Anatomy, 603950, Nizhny Novgorod, Russia.
Klin Lab Diagn. 2019;64(7):405-408. doi: 10.18821/0869-2084-2019-64-7-405-408.
The purpose of the investigation was to study the efficiency of measuring markers of oxidative stress and muscle damage in the oral fluid in highly skilled sportsmen under physical exercise for the assessment of their functional state. 70 highly qualified athletes at the age of 16-20 years specializing in the cyclic kinds of sports (track and field, swimming) took part in the investigation. Sportsmen performed the control test which consisted of the series of 3×100 m distances by a flat race with an active 45 s rest between them for the track and field athletes, and 4×50 m by the main swimming style with an active rest between the distances also for 45 s for the swimmers. Activity of creatine kinase, content of lipid peroxidation products in the blood and oral fluid were measured standard biochemical methods. The performance of the functional tests induces the excessive accumulation of toxic products of lipoperoxidation and increases activity of creatine kinase in the oral fluid of highly qualified athletes. Correlation analysis shows, that the intensity of free radical and peroxide processes in athletes can be evaluated by means of the method of measuring the content of lipid peroxidation products in the oral fluid.
该研究的目的是探讨在体育锻炼过程中，通过检测高水平运动员唾液中氧化应激和肌肉损伤标志物的水平，来评估其功能状态的有效性。70名年龄在16至20岁之间、擅长周期性运动项目（田径、游泳）的高水平运动员参与了此次研究。田径运动员进行的对照测试包括3组100米短跑，每组之间有45秒的积极休息时间；游泳运动员进行的对照测试包括4组50米的主要泳姿，每组之间同样有45秒的积极休息时间。采用标准生化方法测定了血液和唾液中肌酸激酶的活性以及脂质过氧化产物的含量。功能性测试的结果显示，高水平运动员唾液中脂质过氧化有毒产物过度积累，肌酸激酶活性增加。相关性分析表明，通过检测唾液中脂质过氧化产物的含量，可以评估运动员体内自由基和过氧化物反应的强度。
