College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China.
College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China.
Environ Pollut. 2019 Nov;254(Pt A):113022. doi: 10.1016/j.envpol.2019.113022. Epub 2019 Aug 6.
Arsenic (As) present in water is a nonignorable environmental issue, even at low concentrations (≤150 μg L). To evaluate the toxic effect of low concentrations of As, zebrafish at early life stage were exposed to 0, 25, 50, 75, or 150 μg L AsIII for 120 h. Our results indicated that low concentration of AsIII decreased zebrafish larvae's survival rate to 85%, 89% and 86% at 50, 75 and 150 μg L. Furthermore, low concentrations of AsIII exposure caused oxidative stress (elevated superoxide dismutase (SOD) activity and influenced the mRNA transcriptional levels of Cu/ZnSOD and MnSOD) and damage (increased malondialdehyde levels). Meanwhile, zebrafish larvae regulated the mRNA transcription of metallothionein and heat shock protein 70 to alleviate toxicity caused by AsIII. These results revealed lower concentrations (≤150 μg L) of AsIII had a detriment effect on the survival of fish at early life stage, moreover, oxidative stress caused by AsIII posed potential risk for the zebrafish. This study provides novel insight into low concentration AsIII-induced toxicity in zebrafish.
砷（As）即使在低浓度（≤150μg/L）下存在于水中也是一个不容忽视的环境问题。为了评估低浓度砷的毒性作用，本研究将早期发育阶段的斑马鱼暴露于 0、25、50、75 或 150μg/L 的 AsIII 中 120 小时。结果表明，50、75 和 150μg/L 的 AsIII 使斑马鱼幼虫的存活率分别下降至 85%、89%和 86%。此外，低浓度的 AsIII 暴露导致了氧化应激（超氧化物歧化酶（SOD）活性升高和 Cu/ZnSOD 和 MnSOD 的 mRNA 转录水平受到影响）和损伤（丙二醛水平升高）。同时，斑马鱼幼虫调节金属硫蛋白和热休克蛋白 70 的 mRNA 转录，以减轻 AsIII 引起的毒性。这些结果表明，较低浓度（≤150μg/L）的 AsIII 对早期发育阶段鱼类的生存有不利影响，此外，AsIII 引起的氧化应激对斑马鱼构成了潜在风险。本研究为低浓度 AsIII 诱导的斑马鱼毒性提供了新的见解。
