College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang, 321004, PR China.
School of Water Conservancy and Environment, University of Jinan, Jinan, Shandong, 250022, PR China.
Ecotoxicol Environ Saf. 2020 Sep 1;200:110743. doi: 10.1016/j.ecoenv.2020.110743. Epub 2020 May 25.
Gill, as the organ of fish to contact most directly with xenobiotics, suffered more threat. To evaluate the impact of arsenite (AsIII) on the gill of fish, we measured the antioxidative responses (superoxide dismutase (SOD) and catalase (CAT) activities) and oxidative damage (malondialdehyde (MDA) content), histological changes and mRNA transcriptional responses of zebrafish gill, after exposure to AsIII (0, 10, 50, 100, and 150 μg L) solutions for 28 days. We found that AsIII increased the activities of CAT by 46%-87%, decreased the activities of SOD and the contents of MDA by 19% and 21%-32%. Furthermore, CuZnSOD and MnSOD mRNA transcription levels were also inhibited, decreasing by 62%-82% and 70%-77%. Besides, ≥ 100 μg L AsIII also caused histological changes (a loss of mucus and desquamation in the surface of the epithelial cells) on zebrafish gill. These results showed that low concentrations of AsIII influenced biochemical and physiological performances of fish gill, which probably aggravates the toxic effect of AsIII on fish.
鳃作为鱼类与外源性化学物质直接接触的器官，受到了更多的威胁。为了评估亚砷酸盐（AsIII）对鱼类鳃的影响，我们测量了抗氧化反应（超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性）和氧化损伤（丙二醛（MDA）含量）、组织学变化以及斑马鱼鳃的 mRNA 转录反应，在暴露于 AsIII（0、10、50、100 和 150μg/L）溶液 28 天后。我们发现，AsIII 使 CAT 活性增加了 46%至 87%，使 SOD 活性和 MDA 含量降低了 19%和 21%至 32%。此外，CuZnSOD 和 MnSOD 的 mRNA 转录水平也受到抑制，分别降低了 62%至 82%和 70%至 77%。此外，≥100μg/L 的 AsIII 也会导致斑马鱼鳃的组织学变化（上皮细胞表面的黏液丧失和脱落）。这些结果表明，低浓度的 AsIII 影响鱼类鳃的生化和生理性能，这可能会加剧 AsIII 对鱼类的毒性作用。
