College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China.
College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China; Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Science, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Ecotoxicol Environ Saf. 2021 Dec 20;227:112883. doi: 10.1016/j.ecoenv.2021.112883. Epub 2021 Oct 12.
Arsenic (As) in the aquatic environment is a considerable environmental issue, previous studies have reported the toxic effects of low concentrations (≤ 150 μg/L) of As on fish. However, limited information is available regarding the impact of low levels of As on apoptosis. To evaluate this, zebrafish embryos were exposed to different concentrations (0, 25, 50, 75, and 150 μg/L) of As (arsenite [AsIII] and arsenate [AsV]) for 120 h. Our results indicated that low concentrations of AsIII exposure significantly inhibited the survival of zebrafish larvae, and significantly increased the transcription of Caspase-9 and Caspase-3, the ratio of Bax/Bcl-2 transcription, and protein levels of Caspase-3. In contrast, AsV decreased the ratios of Bax/Bcl-2 transcription and protein levels, as well as protein levels of Caspase-3. Our data demonstrated that AsIII and AsV exert different toxic effects, AsIII induced apoptosis via the mitochondrial pathway and the extrinsic pathway, while AsV induced apoptosis only via the mitochondrial pathway.
砷（As）在水环境中是一个相当大的环境问题，先前的研究报告了低浓度（≤150μg/L）的 As 对鱼类的毒性作用。然而，关于低水平的 As 对细胞凋亡的影响的信息有限。为了评估这一点，将斑马鱼胚胎暴露于不同浓度（0、25、50、75 和 150μg/L）的 As（亚砷酸盐[AsIII]和砷酸盐[AsV]）中 120 h。我们的结果表明，低浓度的 AsIII 暴露显著抑制了斑马鱼幼虫的存活，并显著增加了 Caspase-9 和 Caspase-3 的转录、Bax/Bcl-2 转录的比值以及 Caspase-3 的蛋白水平。相比之下，AsV 降低了 Bax/Bcl-2 转录和蛋白水平的比值，以及 Caspase-3 的蛋白水平。我们的数据表明，AsIII 和 AsV 发挥不同的毒性作用，AsIII 通过线粒体途径和外在途径诱导细胞凋亡，而 AsV 仅通过线粒体途径诱导细胞凋亡。
