Department of Chemistry, Sookmyung Women's University, Seoul, 04310, South Korea.
The School of East-West Medical Science, Kyung Hee University, Yongin, 17104, South Korea.
Anal Chim Acta. 2019 Nov 8;1080:153-161. doi: 10.1016/j.aca.2019.07.008. Epub 2019 Jul 9.
A red-emitting and ratiometric fluorescence probe 1 for detecting HO, based on a styrylnaphthalimide-boronate ester was developed. Upon a HO-mediated hydrolysis of boronate ester, probe 1 was transformed to 2 with a ratiometric fluorescence change, decrease at 535 and increase at 640 nm. It was also found that the fluorescent reaction of 1 with HO in solution could be completed within 10 min and the detection limit was estimated to be 0.30 μM. Moreover, this ratiometric change was highly selective for HO over other redox species, metal ions, and anions. Also, this system was found to be capable of detecting HO in the pH range of 6-9. Furthermore, probe 1 was preferentially accumulated into the endoplasmic reticulum (ER) in the live HeLa cells, and an increased HO level in the presence of an ER stress inducer, thapsigargin was revealed.
一种基于苯乙烯萘酰亚胺硼酸酯的红色发射比率荧光探针 1 被开发出来，用于检测 HO。在 HO 介导的硼酸酯水解作用下，探针 1 转化为 2，同时伴随着比率荧光变化，535nm 处的荧光强度降低，640nm 处的荧光强度增加。研究还发现，1 在溶液中与 HO 的荧光反应可以在 10min 内完成，检测限估计为 0.30μM。此外，这种比率变化对 HO 具有高度选择性，优于其他氧化还原物质、金属离子和阴离子。此外，该系统被发现能够在 pH 值为 6-9 的范围内检测 HO。进一步的，探针 1 优先聚集到活 HeLa 细胞的内质网（ER）中，在 ER 应激诱导剂 thapsigargin 的存在下，HO 的水平增加。
