Key Laboratory of Cluster Science of the Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, School of Chemistry, Beijing Institute of Technology , Beijing 100081, People's Republic of China.
Anal Chem. 2017 Dec 5;89(23):12945-12950. doi: 10.1021/acs.analchem.7b03809. Epub 2017 Nov 22.
The endoplasmic reticulum (ER) has a central role in the fine-tuning of environmental and internal stimuli. We herein report a ratiometric fluorescent probe, α-Naph, capable of determining basal HO in the ER. The probe specifically responds to HO. The limit of detection of the probe is as low as 38 nM, making it a feasible sensor to image intracellular basal HO. In addition, utilizing its ratiometric property, we are able to measure the concentration of HO in the ER quantitatively, eliminating the error caused by the probe concentration and environment. The intracellular concentration of HO in the ER is calculated to be 0.692 μM under normal conditions and 1.26 μM under the stimulation of phorbol myristate acetate.
内质网（ER）在精细调节环境和内部刺激方面起着核心作用。我们在此报告了一种比率荧光探针α-Naph，它能够测定 ER 中的基础 HO。该探针特异性地响应 HO。探针的检测限低至 38 nM，使其成为一种可行的传感器，可用于成像细胞内基础 HO。此外，利用其比率特性，我们能够定量测量 ER 中 HO 的浓度，消除探针浓度和环境引起的误差。在正常条件下，细胞内 ER 中 HO 的浓度计算为 0.692 μM，在佛波酯刺激下为 1.26 μM。
