利用姜黄素衍生物（ST06）体外合成的绿色银纳米粒子（ST06-AgNPs）对人宫颈癌（HeLa）细胞和 EAC 荷瘤小鼠模型的影响。

BACKGROUND: In recent years, green synthesized silver nanoparticles have been increasingly investigated for their anti-cancer potential. In the present study, we aimed at the biosynthesis of silver nanoparticles (AgNPs) using a curcumin derivative, ST06. Although, the individual efficacies of silver nanoparticles or curcumin derivatives have been studied previously, the synergistic cytotoxic effects of curcumin derivative and silver nanoparticles in a single nanoparticulate formulation have not been studied earlier specifically on animal models. This makes this study novel compared to the earlier synthesized curcumin derivative or silver nanoparticles studies. The aim of the study was to synthesize ST06 coated silver nanoparticles (ST06-AgNPs) using ST06 as both reducing and coating agent. METHODS: The synthesized nanoparticles AgNPs and ST06-AgNPs were characterised for the particle size distribution, morphology, optical properties and surface charge by using UV-visible spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM). Elemental composition and structural properties were studied by energy dispersive X-ray spectroscopy (EDX) and X-ray diffraction spectroscopy (XRD). The presence of ST06 as capping agent was demonstrated by Fourier transform infrared spectroscopy (FTIR). RESULTS: The synthesized nanoparticles (ST06-AgNPs) were spherical and had a size distribution in the range of 50-100 nm. UV-Vis spectroscopy displayed a specific silver plasmon peak at 410 nm. The in vitro cytotoxicity effects of ST06 and ST06-AgNPs, as assessed by MTT assay, showed significant growth inhibition of human cervical cancer cell line (HeLa). In addition, studies carried out in EAC tumor-induced mouse model (Ehrlich Ascites carcinoma) using ST06-AgNPs, revealed that treatment of the animals with these nanoparticles resulted in a significant reduction in the tumor growth, compared to the control group animals. CONCLUSION: In conclusion, green synthesized ST06-AgNPs exhibited superior anti-tumor efficacy than the free ST06 or AgNPs with no acute toxicity under both in vitro and in vivo conditions. The tumor suppression is associated with the intrinsic apoptotic pathway. Together, the results of this study suggest that ST06-AgNPs could be considered as a potential option for the treatment of solid tumors.

背景：近年来，绿色合成的银纳米粒子因其抗癌潜力而受到越来越多的关注。在本研究中，我们旨在使用姜黄素衍生物 ST06 来合成银纳米粒子（AgNPs）。虽然以前已经研究了银纳米粒子或姜黄素衍生物的单独功效，但在单个纳米颗粒制剂中姜黄素衍生物和银纳米粒子的协同细胞毒性作用以前并未专门在动物模型上进行研究。与以前合成的姜黄素衍生物或银纳米粒子研究相比，这使得本研究具有新颖性。本研究的目的是使用 ST06 作为还原剂和涂层剂来合成 ST06 包裹的银纳米粒子（ST06-AgNPs）。

方法：使用紫外可见分光光度法、动态光散射（DLS）和透射电子显微镜（TEM）对合成的纳米粒子 AgNPs 和 ST06-AgNPs 进行粒径分布、形态、光学性质和表面电荷的表征。通过能量色散 X 射线光谱（EDX）和 X 射线衍射光谱（XRD）研究元素组成和结构性质。通过傅里叶变换红外光谱（FTIR）证明了 ST06 作为包覆剂的存在。

结果：合成的纳米粒子（ST06-AgNPs）为球形，粒径分布在 50-100nm 范围内。紫外可见光谱在 410nm 处显示出特定的银等离子体峰。MTT 测定法评估的 ST06 和 ST06-AgNPs 的体外细胞毒性作用显示，人宫颈癌细胞系（HeLa）的生长受到显著抑制。此外，在 EAC 肿瘤诱导的小鼠模型（Ehrlich 腹水癌）中进行的研究表明，与对照组动物相比，用这些纳米粒子治疗动物可导致肿瘤生长显著减少。

结论：总之，绿色合成的 ST06-AgNPs 在体外和体内条件下均表现出比游离 ST06 或 AgNPs 更高的抗肿瘤功效，且无急性毒性。肿瘤抑制与内在凋亡途径有关。总之，这项研究的结果表明，ST06-AgNPs 可以被认为是治疗实体瘤的一种潜在选择。