Fazylov Serik D, Nurkenov Oralgazy A, Nurmaganbetov Zhangeldy S, Sarsenbekova Akmaral Zh, Bakirova Ryszhan Ye, Seilkhanov Olzhas T, Sviderskiy Alexandr K, Syzdykov Ardak K, Mendibayeva Anel Zh
Institute of Organic Synthesis and Coal Chemistry of the Republic of Kazakhstan, Karaganda 100008, Kazakhstan.
Department of Chemical Technology and Ecology, Karaganda Industrial University, Temirtau 101400, Kazakhstan.
Molecules. 2025 Mar 17;30(6):1337. doi: 10.3390/molecules30061337.
In this study, the synthesis and properties of β-cyclodextrin-functionalized silver nanoparticles and their loading with a drug component are considered. β-Cyclodextrin was used as a reducing agent and stabilizer in the preparation of silver nanoparticles. The use of β-CD-AgNPs in loading molecules of the alkaloid cytisine (Cz) and its O,O-dimethyl-N-cytisinilphosphate (CzP) derivative, which have pronounced antiviral properties, was studied. The formation of β-CD-Cz-AgNPs and β-CD-CzP-AgNPs was confirmed by UV spectroscopy and X-ray diffraction spectroscopy. Scanning electron microscopy and transmission electron microscopy showed that the obtained β-CD-Cz-AgNP and β-CD-CzP-AgNP nanocomposites were well dispersed with particle sizes in the range of 3-20 nm. H-, C-NMR and COSY, HMQC, HMBC and Fourier transform infrared spectroscopy revealed the reduction and encapsulation of AgNPs by β-Cz, and the TEM imaging results showed an increase in the size of nanoparticles after the introduction of cytisine and its phosphorus derivative. The kinetic parameters of the thermal degradation process of β-CD, Cz, CzP and their inclusion complexes Cz(CzP)-β-CD-AgNPs under isothermal conditions, which ensure the preservation of the kinetic triplet, were determined. The differences in the mechanism of thermal decomposition of the studied materials are described by the parameters of the Šesták-Berggren model (m and n), which demonstrated differences for different compounds: for β-CD, the values of the parameters m and n are 0.47 and 0.53, respectively, while for CzP-β-CD-AgNPs they reach values of 0.66 and 1.34. These results indicate differences in the mechanism of thermal decomposition of the studied materials.
本研究考虑了β-环糊精功能化银纳米颗粒的合成、性质及其药物成分负载情况。在银纳米颗粒的制备中，β-环糊精用作还原剂和稳定剂。研究了β-CD-AgNPs对具有显著抗病毒特性的生物碱金雀花碱（Cz）及其O,O-二甲基-N-金雀花碱基磷酸酯（CzP）衍生物分子的负载情况。通过紫外光谱和X射线衍射光谱证实了β-CD-Cz-AgNPs和β-CD-CzP-AgNPs的形成。扫描电子显微镜和透射电子显微镜显示，所获得的β-CD-Cz-AgNP和β-CD-CzP-AgNP纳米复合材料分散良好，粒径在3-20nm范围内。H-、C-NMR以及COSY、HMQC、HMBC和傅里叶变换红外光谱揭示了β-Cz对AgNPs的还原和包封作用，透射电镜成像结果显示引入金雀花碱及其磷衍生物后纳米颗粒尺寸增大。测定了β-CD、Cz、CzP及其包合物Cz(CzP)-β-CD-AgNPs在等温条件下热降解过程的动力学参数，这些参数确保了动力学三重态的保留。通过Šesták-Berggren模型（m和n）的参数描述了所研究材料热分解机制的差异，不同化合物表现出不同：对于β-CD，参数m和n的值分别为0.47和0.53，而对于CzP-β-CD-AgNPs，它们的值分别为0.66和1.34。这些结果表明所研究材料热分解机制存在差异。
