评估寡核苷酸与双链DNA的结合

Assessing Oligonucleotide Binding to Double-Stranded DNA.

作者信息

Mozafari Negin, Umek Tea

机构信息

Department of Laboratory Medicine, Center for Advanced Therapies, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

出版信息

Methods Mol Biol. 2019;2036:91-112. doi: 10.1007/978-1-4939-9670-4_5.

DOI:10.1007/978-1-4939-9670-4_5

PMID:31410792

Abstract

Sequence-specific targeting of double-stranded DNA (dsDNA) using synthetic oligonucleotides (ONs) has been under investigation in different therapeutic approaches. Several methods can be used to evaluate ONs effect and binding capacity to their target sequence. Here we describe some of the methods, which have been frequently used for assessing ONs binding to dsDNA.

摘要

利用合成寡核苷酸（ONs）对双链DNA（dsDNA）进行序列特异性靶向已在不同治疗方法中得到研究。有几种方法可用于评估ONs对其靶序列的作用效果和结合能力。在此，我们描述一些常用于评估ONs与dsDNA结合的方法。