• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

寡核苷酸中 P3'→N5' 膦酸酰胺键的断裂由单链寡核苷酸模板介导。

Cleavage of oligonucleotides containing a P3'→N5' phosphoramidate linkage mediated by single-stranded oligonucleotide templates.

机构信息

Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.

出版信息

Molecules. 2011 Dec 20;16(12):10695-708. doi: 10.3390/molecules161210695.

DOI:10.3390/molecules161210695
PMID:22186956
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6264227/
Abstract

Double-stranded DNA (dsDNA) templates can hybridize to and accelerate cleavage of oligonucleotides containing a P3'→N5' phosphoramidate (P-N) linkage. This dsDNA-templated cleavage of P-N linkages could be due to conformational strain placed on the linkage upon triplex formation. To determine whether duplex formation also induced conformational strain, we examined the reactivity of the oligonucleotides with a P-N linkage in the presence of single-stranded templates, and compared these reactions to those with dsDNA templates. P-N oligonucleotides that are cleaved upon duplex formation could be used as probes to detect single-stranded nucleic acids.

摘要

双链 DNA (dsDNA) 模板可以杂交并加速含有 P3'→N5' 膦酰胺酸酯 (P-N) 键的寡核苷酸的切割。这种 dsDNA 模板切割 P-N 键可能是由于三链体形成时在键上产生的构象应变。为了确定双链体形成是否也会引起构象应变，我们研究了在单链模板存在下具有 P-N 键的寡核苷酸的反应性，并将这些反应与具有 dsDNA 模板的反应进行了比较。在双链体形成时被切割的 P-N 寡核苷酸可用作探针来检测单链核酸。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/b007494292a8/molecules-16-10695-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/eb89b810f2ad/molecules-16-10695-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/68817d89e2fe/molecules-16-10695-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/28a9e21d6fae/molecules-16-10695-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/a8a314410614/molecules-16-10695-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/eade6010990b/molecules-16-10695-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/b196ea221975/molecules-16-10695-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/b007494292a8/molecules-16-10695-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/eb89b810f2ad/molecules-16-10695-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/68817d89e2fe/molecules-16-10695-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/28a9e21d6fae/molecules-16-10695-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/a8a314410614/molecules-16-10695-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/eade6010990b/molecules-16-10695-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/b196ea221975/molecules-16-10695-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907e/6264227/b007494292a8/molecules-16-10695-g006.jpg

相似文献

1
Cleavage of oligonucleotides containing a P3'→N5' phosphoramidate linkage mediated by single-stranded oligonucleotide templates.寡核苷酸中 P3'→N5' 膦酸酰胺键的断裂由单链寡核苷酸模板介导。
Molecules. 2011 Dec 20;16(12):10695-708. doi: 10.3390/molecules161210695.
2
Acid-mediated cleavage of oligonucleotide P3' --> N5' phosphoramidates triggered by sequence-specific triplex formation.由序列特异性三链体形成引发的酸介导的寡核苷酸P3'→N5'氨基磷酸酯的切割。
Nucleosides Nucleotides Nucleic Acids. 2007;26(8-9):893-6. doi: 10.1080/15257770701505741.
3
Synthesis and properties of 5'-amino-2',4'-BNA modified oligonucleotides with P3'-->N5' phosphoramidate linkages.具有3'-磷酰胺基-5'-氮基连接的5'-氨基-2',4'-双环核苷酸修饰寡核苷酸的合成与性质
Nucleic Acids Res Suppl. 2002(2):25-6. doi: 10.1093/nass/2.1.25.
4
Adjustment of the gamma dihedral angle of an oligonucleotide P3'-->N5' phosphoramidate enhances its binding affinity towards complementary strands.寡核苷酸P3'→N5'氨基磷酸酯的γ二面角的调整增强了其对互补链的结合亲和力。
Angew Chem Int Ed Engl. 2005 Mar 18;44(13):1944-7. doi: 10.1002/anie.200461942.
5
Matrix-induced fragmentation of P3'-N5' phosphoramidate-containing DNA: high-throughput MALDI-TOF analysis of genomic sequence polymorphisms.基质诱导含3'-N5'磷酰胺酸的DNA片段化：基因组序列多态性的高通量基质辅助激光解吸电离飞行时间分析
Nucleic Acids Res. 2001 Sep 15;29(18):3864-72. doi: 10.1093/nar/29.18.3864.
6
Double-stranded DNA-templated cleavage of oligonucleotides containing a P3'->N5' linkage triggered by triplex formation: the effects of chemical modifications and remarkable enhancement in reactivity.双链 DNA 模板引导的寡核苷酸断裂，其中包含 P3'->N5' 键，由三链体形成触发：化学修饰的影响和反应性的显著增强。
Nucleic Acids Res. 2010 Nov;38(20):7332-42. doi: 10.1093/nar/gkq600. Epub 2010 Jul 8.
7
Synthesis and properties of a novel bridged nucleic acid analogue bearing a P3'-->N5' phosphoroamidate linkage, 5'-amino-3', 5'-BNA.一种带有P3'→N5'氨基磷酸酯键的新型桥连核酸类似物5'-氨基-3', 5'-BNA的合成与性质
Nucleic Acids Symp Ser (Oxf). 2004(48):7-8. doi: 10.1093/nass/48.1.7.
8
Promotion of acid-mediated cleavage of oligonucleotide P3'->N5' phosphoroamidates by triplex formation: a novel approach to sequence-specific DNA detection.通过三链体形成促进酸介导的寡核苷酸P3'→N5'氨基磷酸酯的切割：一种序列特异性DNA检测的新方法。
Nucleic Acids Symp Ser (Oxf). 2005(49):157-8. doi: 10.1093/nass/49.1.157.
9
Double-stranded DNA-templated oligonucleotide digestion triggered by triplex formation.
Chembiochem. 2007 Nov 5;8(16):1924-8. doi: 10.1002/cbic.200700430.
10
Highly stable DNA triplexes formed with cationic phosphoramidate pyrimidine alpha-oligonucleotides.由阳离子氨基磷酸嘧啶α-寡核苷酸形成的高度稳定的DNA三链体。
Chembiochem. 2005 Jul;6(7):1254-62. doi: 10.1002/cbic.200400436.

引用本文的文献

1
Oligonucleotide-templated reactions for sensing nucleic acids.寡核苷酸模板反应在核酸检测中的应用。
Molecules. 2012 Feb 29;17(3):2446-63. doi: 10.3390/molecules17032446.

本文引用的文献

1
Effect of locked nucleic acid modifications on the thermal stability of noncanonical DNA structure.锁核酸修饰对非 canonical DNA 结构热稳定性的影响。
Biochemistry. 2011 Aug 30;50(34):7414-25. doi: 10.1021/bi200477g. Epub 2011 Aug 8.
2
A fluorogenic reaction based on heavy-atom removal for ultrasensitive DNA detection.基于重原子去除的荧光反应用于超灵敏 DNA 检测。
J Am Chem Soc. 2010 Sep 8;132(35):12197-9. doi: 10.1021/ja105181v.
3
Double-stranded DNA-templated cleavage of oligonucleotides containing a P3'->N5' linkage triggered by triplex formation: the effects of chemical modifications and remarkable enhancement in reactivity.
双链 DNA 模板引导的寡核苷酸断裂，其中包含 P3'->N5' 键，由三链体形成触发：化学修饰的影响和反应性的显著增强。
Nucleic Acids Res. 2010 Nov;38(20):7332-42. doi: 10.1093/nar/gkq600. Epub 2010 Jul 8.
4
Efficient nucleic acid detection by templated reductive quencher release.通过模板还原抑制剂释放实现高效核酸检测。
J Am Chem Soc. 2009 Nov 11;131(44):16021-3. doi: 10.1021/ja904138v.
5
Contribution of the intrinsic mechanical energy of the phosphodiester linkage to the relative stability of the A, BI, and BII forms of duplex DNA.磷酸二酯键的内在机械能对双链DNA的A、BI和BII形式相对稳定性的贡献。
J Phys Chem B. 2009 Mar 12;113(10):3235-44. doi: 10.1021/jp8102782.
6
Nucleic acid templated reactions: consequences of probe reactivity and readout strategy for amplified signaling and sequence selectivity.核酸模板反应：探针反应性及读出策略对信号放大和序列选择性的影响
Chemistry. 2009 Jul 6;15(27):6723-30. doi: 10.1002/chem.200900025.
7
Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences.用于寡核苷酸序列双色检测的还原触发红色荧光探针。
Org Biomol Chem. 2009 Feb 21;7(4):671-7. doi: 10.1039/b817228e. Epub 2008 Dec 12.
8
7-Azidomethoxy-coumarins as profluorophores for templated nucleic acid detection.7-叠氮甲氧基香豆素作为用于模板核酸检测的前体荧光团。
Chembiochem. 2008 Dec 15;9(18):2981-8. doi: 10.1002/cbic.200800507.
9
Target-catalyzed transfer reactions for the amplified detection of RNA.用于RNA扩增检测的靶标催化转移反应
Angew Chem Int Ed Engl. 2008;47(37):7119-22. doi: 10.1002/anie.200801355.
10
Organometallic activation of a fluorogen for templated nucleic acid detection.用于模板核酸检测的荧光团的有机金属活化
Org Lett. 2008 Jul 17;10(14):2935-8. doi: 10.1021/ol800878b. Epub 2008 Jun 13.