Ruvolo Giovanni, Roccheri Maria Carmela, Luparello Claudio, Matranga Domenica, Ferrigno Alberto, Bosco Liana
Centro di Biologia della Riproduzione, Via Villareale Palermo, Sicily.
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche. Università degli Studi di Palermo, Viale delle Scienze Ed.16, Palermo, Sicily.
Zygote. 2019 Oct;27(5):350-354. doi: 10.1017/S0967199419000248. Epub 2019 Aug 14.
Activated pERK1/2 and pAKT are key players in supporting cell survival and proliferation pathways. Translocation of pERK1/2 into the nucleus, where it interacts with transcription factors and DNA itself, is instrumental in exerting an anti-apoptotic effect. In this study, pAKT levels, pERK1/2 nuclear localization and DNA fragmentation index (DFI) in cumulus cells of single cumulus-oocyte complexes of patients undergoing in vitro fertilization programmes were evaluated and correlated with the clinical outcome of the related embryos. For a positive clinical outcome of blastocyst development, pERK1/2 nuclear localization and DFI value had a significant inverse relationship, whereas the former and the intracellular accumulation of pAKT had a significant direct relationship. This relationship was not observed for the negative clinical outcome of the arrested embryos. Moreover, intracellular accumulation of pAKT and DFI value had a significant inverse relationship in all samples examined. The obtained data suggest that the intranuclear relocation of pERK1/2, along with an enhanced intracellular accumulation of pAKT, may exert a survival effect and increase cell viability, therefore providing a novel marker tool to choose the best oocyte to be fertilized and submitted to an intracytoplasmic sperm injection cycle.
活化的pERK1/2和pAKT是支持细胞存活和增殖途径的关键因子。pERK1/2易位至细胞核，在细胞核中它与转录因子及DNA本身相互作用，这对发挥抗凋亡作用至关重要。在本研究中，对接受体外受精程序患者的单个卵丘-卵母细胞复合体的卵丘细胞中的pAKT水平、pERK1/2核定位及DNA片段化指数（DFI）进行了评估，并将其与相关胚胎的临床结局相关联。对于囊胚发育的阳性临床结局，pERK1/2核定位与DFI值呈显著负相关，而前者与pAKT的细胞内积累呈显著正相关。对于胚胎停滞的阴性临床结局，未观察到这种关系。此外，在所有检测样本中，pAKT的细胞内积累与DFI值呈显著负相关。所得数据表明，pERK1/2的核内重新定位以及pAKT细胞内积累的增强可能发挥存活效应并增加细胞活力，因此提供了一种新型标记工具，用于选择最佳的卵母细胞进行受精并用于胞浆内单精子注射周期。
