Nurminen M, Karvonen M, Siitonen A
National Public Health Institute, Helsinki, Finland.
Eur J Clin Microbiol Infect Dis. 1988 Aug;7(4):529-31. doi: 10.1007/BF01962607.
The value of the chromogenic limulus assay for detection of gram-negative bacteria in urine was determined. The assay was performed in microtiter plates at room temperature. In 311 consecutively collected urine samples from patients with suspected urinary tract infection, the assay was positive in all 35 samples containing greater than or equal to 10(5) bacteria/ml. No false positive or false negative results were obtained. Four of six urine specimens from patients with gonococcal infection were positive in the assay, whereas samples from patients with chlamydial infection did not yield a positive result. The assay is a rapid and reliable method for detection of urinary tract infection caused by gram-negative bacteria when greater than or equal to 10(5) bacteria/ml are present.
测定了用于检测尿液中革兰氏阴性菌的显色鲎试剂检测法的价值。该检测法在室温下于微量滴定板中进行。在311份连续收集的疑似尿路感染患者的尿液样本中，所有每毫升含大于或等于10⁵ 个细菌的35份样本检测法均呈阳性。未获得假阳性或假阴性结果。6份淋球菌感染患者的尿液标本中有4份检测法呈阳性，而衣原体感染患者的样本未产生阳性结果。当每毫升存在大于或等于10⁵ 个细菌时，该检测法是检测由革兰氏阴性菌引起的尿路感染的快速且可靠的方法。
