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1
Detection of endotoxin in the plasma of patients with gram-negative bacterial sepsis by the Limulus amoebocyte lysate assay.应用鲎试剂法检测革兰阴性菌败血症患者血浆中的内毒素。
J Clin Microbiol. 1985 Jun;21(6):865-8. doi: 10.1128/jcm.21.6.865-868.1985.
2
A novel endotoxin-specific assay by turbidimetry with Limulus amoebocyte lysate containing beta-glucan.一种采用含有β-葡聚糖的鲎试剂通过比浊法进行的新型内毒素特异性检测方法。
J Biochem Biophys Methods. 1991 Feb-Mar;22(2):93-100. doi: 10.1016/0165-022x(91)90022-o.
3
[Clinical value of endotoxin determination in infection. Comparison of the Limulus amebocyte lysate test with detection of bacterial pathogens].[感染中内毒素测定的临床价值。鲎试剂检测与细菌病原体检测的比较]
Acta Med Austriaca. 1987;14(1):21-4.
4
Assay of endotoxin in human plasma using immobilized histidine, Limulus amoebocyte lysate and chromogenic substrate.使用固定化组氨酸、鲎试剂和显色底物测定人血浆中的内毒素
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Gel clot LAL assay in the initial management of peritoneal dialysis patients with peritonitis: a retrospective study.凝胶凝块鲎试剂检测法在腹膜炎腹膜透析患者初始治疗中的应用：一项回顾性研究
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Limulus amebocyte lysate test for endotoxemia: investigations with a femtogram sensitive spectrophotometric assay.鲎试剂检测内毒素血症：飞克灵敏度分光光度法研究
Klin Wochenschr. 1981 Mar 2;59(5):213-8. doi: 10.1007/BF01476578.

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Eur J Clin Microbiol Infect Dis. 2010 Feb;29(2):207-15. doi: 10.1007/s10096-009-0841-2. Epub 2009 Nov 29.
8
Diagnosis of endotoxemia with gram-negative bacteremia is bacterial species dependent: a meta-analysis of clinical studies.革兰氏阴性菌菌血症患者的内毒素血症诊断依赖于细菌种类：临床研究的荟萃分析。
J Clin Microbiol. 2009 Dec;47(12):3826-31. doi: 10.1128/JCM.01189-09. Epub 2009 Sep 30.
9
Concordance of bacterial cultures with endotoxin and interleukin-6 in necrotizing enterocolitis.坏死性小肠结肠炎中细菌培养与内毒素及白细胞介素-6的一致性
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10
Detection of endotoxin using an evanescent wave fiber-optic biosensor.使用倏逝波光光纤生物传感器检测内毒素。
Appl Biochem Biotechnol. 1996 Sep;60(3):189-202. doi: 10.1007/BF02783583.

本文引用的文献

1
Potentiating effect of calcium gluconate on the Limulus amebocyte lysate (LAL) gelation-endpoint assay for endotoxin.葡萄糖酸钙对内毒素鲎试剂凝胶化终点检测的增强作用。
J Parenter Sci Technol. 1981 Sep-Oct;35(5):242-7.
2
The use of LAL as an alternative to the CFR rabbit pyrogen test for disodium ticarcillin.使用鲎试剂替代羧苄西林二钠的家兔热原试验。
Prog Clin Biol Res. 1982;93:91-100.
3
A corporate approach to in-process and end-product testing with the LAL assay for endotoxin.一种采用鲎试剂法进行内毒素过程中和终产品检测的企业方法。
Prog Clin Biol Res. 1982;93:231-46.
4
[Studies on the gelation reaction of Limulus lysate (pre-gel). V. Purification of the inhibitory substances of the gelation reaction of Limulus lysate from human plasma (author's transl)].[鲎溶解物（凝胶前体）凝胶化反应的研究。V. 从人血浆中纯化鲎溶解物凝胶化反应的抑制物质（作者译）]
Yakugaku Zasshi. 1980 May;100(5):500-8. doi: 10.1248/yakushi1947.100.5_500.
5
Influence of fine structure of lipid A on Limulus amebocyte lysate clotting and toxic activities.脂多糖A的精细结构对鲎试剂凝血及毒性活性的影响。
Infect Immun. 1984 Aug;45(2):350-5. doi: 10.1128/iai.45.2.350-355.1984.
6
New limulus amoebocyte lysate test for endotoxaemia.用于内毒素血症的新型鲎试剂检测法。
Lancet. 1982 Jan 30;1(8266):289. doi: 10.1016/s0140-6736(82)91016-9.
7
Detection of endotoxin in human blood and demonstration of an inhibitor.人体血液中内毒素的检测及一种抑制剂的证明
J Lab Clin Med. 1970 Jun;75(6):903-11.
8
Host defense against bacterial endotoxemia: mechanism in normal animals.宿主对细菌内毒素血症的防御：正常动物中的机制。
J Exp Med. 1970 Aug 1;132(2):300-16. doi: 10.1084/jem.132.2.300.
9
Clearance of endotoxin by platelets: role in increasing the accuracy of the Limulus gelation test and in combating experimental endotoxemia.血小板对内毒素的清除作用：在提高鲎试剂凝胶试验准确性及对抗实验性内毒素血症中的作用。
Surgery. 1973 Aug;74(2):235-40.
10
Opsonizing antibodies, host factors, and the limulus assay for endotoxin.调理素抗体、宿主因素及内毒素鲎试剂检测法
Infect Immun. 1975 Jul;12(1):88-92. doi: 10.1128/iai.12.1.88-92.1975.

应用鲎试剂法检测革兰阴性菌败血症患者血浆中的内毒素。

Detection of endotoxin in the plasma of patients with gram-negative bacterial sepsis by the Limulus amoebocyte lysate assay.

作者信息

Pearson F C, Dubczak J, Weary M, Bruszer G, Donohue G

出版信息

J Clin Microbiol. 1985 Jun;21(6):865-8. doi: 10.1128/jcm.21.6.865-868.1985.

DOI:10.1128/jcm.21.6.865-868.1985
PMID:4008617
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271807/
Abstract

A total of 120 Limulus amoebocyte lysate (LAL) determinations were made on plasma obtained from normal, healthy human blood donors. Results demonstrated a mean endotoxin level in blood of 0.02 to 1.57 pg/ml. The amount of Escherichia coli endotoxin added to human plasma samples can be quantitated by both nephelometry and turbidimetry. Endotoxin-spiked samples were shown to be significantly different from unspiked samples. When plasma samples were collected from 45 patients hospitalized at three centers, a strong association was demonstrated between a positive Limulus amoebocyte lysate assay and a septic condition. Sensitivity, specificity, and false-positive and false-negative rates for the Limulus amoebocyte lysate assay as a diagnostic test for gram-negative bacteremia were estimated.

摘要

对从正常、健康的人类献血者采集的血浆进行了总共120次鲎试剂（LAL）检测。结果显示血液中的内毒素平均水平为0.02至1.57 pg/ml。添加到人血浆样本中的大肠杆菌内毒素量可以通过比浊法和浊度法进行定量。添加内毒素的样本与未添加内毒素的样本有显著差异。当从三个中心住院的45名患者采集血浆样本时，鲎试剂检测呈阳性与脓毒症状态之间存在很强的关联。估计了鲎试剂检测作为革兰氏阴性菌血症诊断试验的敏感性、特异性、假阳性率和假阴性率。