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使用自动鲎试剂检测法快速检测显著菌尿症。

Rapid detection of significant bacteriuria by use of an automated Limulus amoebocyte lysate assay.

作者信息

Jorgensen J H, Alexander G A

出版信息

J Clin Microbiol. 1982 Sep;16(3):587-9. doi: 10.1128/jcm.16.3.587-589.1982.

DOI:10.1128/jcm.16.3.587-589.1982
PMID:7130374
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272421/
Abstract

Previous studies have demonstrated that significant gram-negative bacteriuria can be detected by using the Limulus amoebocyte lysate test. A series of 580 urine specimens were tested in parallel with the automated MS-2 (Abbott Laboratories) assay and with quantitative urine bacterial cultures. The overall ability of the MS-2 Limulus amoebocyte lysate test to correctly classify urine specimens as containing either greater than or equal to 10(5) organisms or less than 10(5) organisms per ml during a 20-min test period was 92.6%.

摘要

先前的研究表明,使用鲎试剂检测法可以检测到显著的革兰氏阴性菌尿。对580份尿液标本进行了一系列检测,同时采用自动化MS-2(雅培实验室)检测法和定量尿液细菌培养法。在20分钟的检测期内,MS-2鲎试剂检测法将尿液标本正确分类为每毫升含有大于或等于10⁵个细菌或少于10⁵个细菌的总体能力为92.6%。

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引用本文的文献

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Use of the Bac-T-Screen to predict bacteriuria from urine specimens held at room temperature.使用Bac-T-Screen检测法预测室温保存尿液标本中的菌尿症。
J Clin Microbiol. 1986 Aug;24(2):214-7. doi: 10.1128/jcm.24.2.214-217.1986.

本文引用的文献

1
EVALUATION OF THE TETRAZOLIUM TEST FOR THE DIAGNOSIS OF SIGNIFICANT BACTERIURIA.用于诊断显著菌尿症的四氮唑试验评估
JAMA. 1965 May 31;192:769. doi: 10.1001/jama.1965.03080220033007.
2
SIMPLE TESTS FOR SIGNIFICANT BACTERIURIA.显著菌尿的简易检测方法
Arch Intern Med. 1963 Nov;112:742-6. doi: 10.1001/archinte.1963.03860050129015.
3
Automation of the Limulus amoebocyte lysate test by using the Abbott MS-2 microbiology system.使用雅培MS-2微生物学系统对鲎试剂检测进行自动化操作。
Appl Environ Microbiol. 1981 Jun;41(6):1316-20. doi: 10.1128/aem.41.6.1316-1320.1981.
4
Screening of urine cultures by three automated systems.通过三种自动化系统对尿培养进行筛查。
J Clin Microbiol. 1982 Mar;15(3):468-74. doi: 10.1128/jcm.15.3.468-474.1982.
5
Identification of elements of urinary sediment with phase-contrast microscopy. A simple method.利用相差显微镜识别尿沉渣成分。一种简单的方法。
JAMA. 1968 Nov 18;206(8):1777-81.
6
Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay.鲎试剂用于内毒素检测的制备、灵敏度和特异性。
Appl Microbiol. 1973 Jul;26(1):43-8. doi: 10.1128/am.26.1.43-48.1973.
7
Comparative evaluation of the Limulus assay and the direct Gram stain for detection of significant bacteriuria.鲎试剂检测法与直接革兰氏染色法检测显著菌尿症的比较评估
Am J Clin Pathol. 1975 Jan;63(1):142-8. doi: 10.1093/ajcp/63.3.142.