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使用自动鲎试剂检测法快速检测显著菌尿症。

Rapid detection of significant bacteriuria by use of an automated Limulus amoebocyte lysate assay.

作者信息

Jorgensen J H, Alexander G A

出版信息

J Clin Microbiol. 1982 Sep;16(3):587-9. doi: 10.1128/jcm.16.3.587-589.1982.

Abstract

Previous studies have demonstrated that significant gram-negative bacteriuria can be detected by using the Limulus amoebocyte lysate test. A series of 580 urine specimens were tested in parallel with the automated MS-2 (Abbott Laboratories) assay and with quantitative urine bacterial cultures. The overall ability of the MS-2 Limulus amoebocyte lysate test to correctly classify urine specimens as containing either greater than or equal to 10(5) organisms or less than 10(5) organisms per ml during a 20-min test period was 92.6%.

摘要

先前的研究表明,使用鲎试剂检测法可以检测到显著的革兰氏阴性菌尿。对580份尿液标本进行了一系列检测,同时采用自动化MS-2(雅培实验室)检测法和定量尿液细菌培养法。在20分钟的检测期内,MS-2鲎试剂检测法将尿液标本正确分类为每毫升含有大于或等于10⁵个细菌或少于10⁵个细菌的总体能力为92.6%。

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