College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China.
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
Transbound Emerg Dis. 2020 Jan;67(1):6-10. doi: 10.1111/tbed.13331. Epub 2019 Aug 28.
Circoviruses are found in many species, including mammals, birds, lower vertebrates and invertebrates. To date, there are no reports of circovirus-induced diseases in chickens. In this study, we identified a new strain of chicken circovirus (CCV) by PacBio third-generation sequencing samples from chickens with acute gastroenteritis in a Shandong commercial broiler farm in China. The complete genome of CCV was verified by inverse PCR. Genomic analysis revealed that CCV codes two inverse open reading frames (ORFs), and a potential stem-loop structure was present at the 5' end with a structure typical of a circular virus. Phylogenetic tree analysis showed that CCV formed an independent branch between mammalian and avian circovirus, and homology analysis indicated that the homology of CCV with 21 other known circoviruses was less than 40%. Thus, this CCV strain represents a new species in the genus Circovirus. The infection rate of CCV in 12 chickens with diarrhoea was 100%, but no CCV was found in healthy chickens, thereby indicating that the novel CCV strain is highly associated with acute infectious gastroenteritis in chickens. The emergence of a novel CCV in commercial broiler chickens is highly concerning for the broiler industry.
圆环病毒存在于许多物种中，包括哺乳动物、鸟类、低等脊椎动物和无脊椎动物。迄今为止，尚未有关于圆环病毒引起鸡病的报道。在本研究中，我们通过在中国山东一家商业肉鸡养殖场患有急性肠胃炎的鸡的 PacBio 第三代测序样本中鉴定出一种新型鸡圆环病毒（CCV）。通过反向 PCR 验证了 CCV 的完整基因组。基因组分析表明，CCV 编码两个反向开放阅读框（ORF），5'端存在潜在的茎环结构，具有环状病毒的典型结构。系统进化树分析显示，CCV 在哺乳动物和禽类圆环病毒之间形成一个独立的分支，同源性分析表明 CCV 与其他 21 种已知圆环病毒的同源性小于 40%。因此，该 CCV 株代表圆环病毒属的一个新种。在 12 只腹泻鸡中，CCV 的感染率为 100%，但在健康鸡中未发现 CCV，表明新型 CCV 株与鸡急性传染性肠胃炎高度相关。新型 CCV 在商业肉鸡鸡群中的出现，对肉鸡产业高度关注。
