Wei Xi Chao, Lv Zhong Hua
Department of Urology, Jining Hospital of Traditional Chinese Medicine, Jining 272000, Shandong, People's Republic of China.
Department of Urology, Jining No. 1 People's Hospital, Jining 272011, Shandong, People's Republic of China.
Onco Targets Ther. 2019 Jul 23;12:5937-5945. doi: 10.2147/OTT.S201731. eCollection 2019.
Increasing evidence shows that microRNAs play an important regulatory role in the development of several types of cancers. However, the role of microRNA-132 (miR-132) in human bladder cancer (BC) metastasis remains unclear. In this research, we aimed to investigate the effect of miR-132 on the cell migration and relate potential mechanism in BC. miR-132 expression level was assessed by quantitative real-time PCR (qRT-PCR) in 32 BC tissues and BC cell lines (T24). The function of miR-132 was evaluated by Transwell assay. Gene expression was determined by using qRT-PCR or Western blot. The results showed that miR-132 had a lower expression in BC tissues than in adjacent normal tissues. At the same time, compared to human normal urethral epithelium cells, the expression level of miR-132 was downregulated in T24 cell lines. miR-132 overexpression significantly inhibited migration and invasion capacities in T24 cells, while downregulation of miR-132 expression strengthened such capacities. Compared with those transfected with miR-132 mimic, EMT-related markers and TGFβ1/Smad2 expression levels were higher in T24 cells transfected with miR-132 inhibitor. Moreover, EMT-related markers and Smad2 expression levels was obviously increased in BC tissues compared to the adjacent normal tissues. The correlation result indicated that the expression of miR-132 and Smad2 was reversed. In short, our results suggest that miR-132 may play a suppressive role in the metastasis of BC cells via TGFβ1/Smad2 signaling pathway.
越来越多的证据表明，微小RNA在多种癌症的发展中发挥着重要的调节作用。然而，微小RNA - 132（miR - 132）在人类膀胱癌（BC）转移中的作用仍不清楚。在本研究中，我们旨在探讨miR - 132对BC细胞迁移的影响及其潜在机制。通过定量实时PCR（qRT - PCR）评估了32例BC组织和BC细胞系（T24）中miR - 132的表达水平。通过Transwell实验评估miR - 132的功能。使用qRT - PCR或蛋白质免疫印迹法测定基因表达。结果显示，miR - 132在BC组织中的表达低于相邻正常组织。同时，与人类正常尿道上皮细胞相比，miR - 132在T24细胞系中的表达水平下调。miR - 132过表达显著抑制T24细胞的迁移和侵袭能力，而miR - 132表达下调则增强了这些能力。与转染miR - 132模拟物的细胞相比，转染miR - 132抑制剂的T24细胞中EMT相关标志物和TGFβ1/Smad2表达水平更高。此外，与相邻正常组织相比，BC组织中EMT相关标志物和Smad2表达水平明显升高。相关性结果表明，miR - 132和Smad2的表达呈负相关。简而言之，我们的结果表明，miR - 132可能通过TGFβ1/Smad2信号通路在BC细胞转移中发挥抑制作用。
