Ankenbauer R G, Cox C D
Department of Microbiology, University of Iowa, Iowa City 52242.
J Bacteriol. 1988 Nov;170(11):5364-7. doi: 10.1128/jb.170.11.5364-5367.1988.
Pseudomonas aeruginosa mutants requiring salicylic acid for pyochelin biosynthesis were isolated after chemical mutagenesis by plating on a siderophore detection medium. Like the wild type, these mutants incorporated 7-[14C]salicylic acid into pyochelin, demonstrating that salicylic acid is an intermediate in the biosynthesis pathway of pyochelin.
通过在铁载体检测培养基上平板接种,在化学诱变后分离出了在绿脓菌素生物合成中需要水杨酸的铜绿假单胞菌突变体。与野生型一样,这些突变体将7-[¹⁴C]水杨酸掺入绿脓菌素中,表明水杨酸是绿脓菌素生物合成途径中的中间体。