Diettrich Jan, Kage Hirokazu, Nett Markus
Department of Biochemical and Chemical Engineering, Laboratory of Technical Biology, TU Dortmund University, Emil-Figge-Strasse 66, 44227 Dortmund, Germany.
Beilstein J Org Chem. 2019 Jun 13;15:1298-1303. doi: 10.3762/bjoc.15.128. eCollection 2019.
A putative siderophore locus was detected in the genome of the violacein-producing bacterium sp. NR 4-1 and predicted to direct the biosynthesis of a molecule that is structurally related to the thiazoline-containing siderophore micacocidin. In order to track this compound, we analyzed the metabolic profiles of cultures grown under different iron concentrations. A compound which was found to be predominantly produced under iron deficiency was subsequently isolated. Its structural characterization by spectroscopic and bioinformatic analyses revealed a previously not known diastereomer of the cytotoxic alkaloid agrochelin. The structure of this natural product, which was named massiliachelin, corresponds to the assembly line encoded by the identified siderophore locus.
在产紫菌素的细菌sp. NR 4-1基因组中检测到一个假定的铁载体基因座，预计该基因座可指导一种与含噻唑啉铁载体米卡可西定结构相关的分子的生物合成。为了追踪这种化合物，我们分析了在不同铁浓度下培养的代谢谱。随后分离出一种在缺铁条件下主要产生的化合物。通过光谱和生物信息学分析对其结构进行表征，揭示了一种以前未知的细胞毒性生物碱农杆菌素的非对映异构体。这种天然产物被命名为马赛菌素，其结构与所鉴定的铁载体基因座编码的装配线相对应。
