绿脓菌素：铜绿假单胞菌铁螯合生长促进剂的新结构
Pyochelin: novel structure of an iron-chelating growth promoter for Pseudomonas aeruginosa.
作者信息
Cox C D, Rinehart K L, Moore M L, Cook J C
出版信息
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4256-60. doi: 10.1073/pnas.78.7.4256.
Abstract
Pyochelin, an endogenous growth promoter that solubilizes ferric iron, has been isolated from Pseudomonas aeruginosa, including clinical strains. The structure of pyochelin has been assigned as 2-(2-o-hydroxyphenyl-2-thiazolin-4-yl)-3-methylthiazolidine-4-carboxylic acid and is of a different type from those previously assigned to siderophores (siderochromes) from bacteria. The assignment rests on 1H and 13C NMR data, high-resolution (including field desorption) mass spectrometry, and spectroscopic properties of synthetic model compounds. Pyochelin is presumed to be biosynthesized from salicylic acid and two moles of cysteine.
摘要
绿脓菌素是一种可溶解三价铁的内源性生长促进剂，已从铜绿假单胞菌（包括临床菌株）中分离出来。绿脓菌素的结构已确定为2-(2-邻羟基苯基-2-噻唑啉-4-基)-3-甲基噻唑烷-4-羧酸，与先前确定的细菌铁载体（铁色素）类型不同。该结构的确定基于氢核磁共振（1H NMR）和碳核磁共振（13C NMR）数据、高分辨率（包括场解吸）质谱以及合成模型化合物的光谱特性。据推测，绿脓菌素是由水杨酸和两摩尔半胱氨酸生物合成的。
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