Center for Systems and Synthetic Biology, Institute of Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA.
Center for Systems and Synthetic Biology, Institute of Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA.
Trends Biotechnol. 2020 Feb;38(2):154-162. doi: 10.1016/j.tibtech.2019.06.001. Epub 2019 Aug 12.
The spatial distribution of molecules and cells is fundamental to understanding biological systems. Traditionally, microscopies based on electromagnetic waves such as visible light have been used to localize cellular components by direct visualization. However, these techniques suffer from limitations of transmissibility and throughput. Complementary to optical approaches, biochemical techniques such as crosslinking can colocalize molecules without suffering the same limitations. However, biochemical approaches are often unable to combine individual colocalizations into a map across entire cells or tissues. Microscopy-by-sequencing techniques aim to biochemically colocalize DNA-barcoded molecules and, by tracking their thus unique identities, reconcile all colocalizations into a global spatial map. Here, we review this new field and discuss its enormous potential to answer a broad spectrum of questions.
分子和细胞的空间分布对于理解生物系统至关重要。传统上，基于电磁波（如可见光）的显微镜被用于通过直接可视化来定位细胞成分。然而，这些技术受到传输和通量的限制。与光学方法互补的是，交联等生化技术可以对分子进行共定位，而不会受到相同的限制。然而，生化方法通常无法将单个共定位组合成整个细胞或组织的图谱。基于测序的显微镜技术旨在对 DNA 编码分子进行生化共定位，并通过跟踪其独特的身份，将所有共定位整合到一个全局空间图谱中。在这里，我们回顾了这个新领域，并讨论了它在回答广泛问题方面的巨大潜力。
