低级别胶质瘤中免疫相关 lncRNA 的生存分析。
Survival analysis of immune-related lncRNA in low-grade glioma.
机构信息
Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, China.
Department of Stomatology, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Shenyang, Liaoning Province, 110004, People's Republic of China.
出版信息
BMC Cancer. 2019 Aug 16;19(1):813. doi: 10.1186/s12885-019-6032-3.
BACKGROUND
Low-grade glioma is grade I-II glioma. Immunotherapy is a promising way of tumor killing. Research on immune molecular mechanisms in low-grade gliomas and discovery of new immune checkpoints for low-grade gliomas are of great importance.
METHODS
Gene sequencing data and clinical data of low-grade glioma were downloaded from TCGA database. Prognosis related lncRNAs were identified by Cox regression and their possible functions were found by gene enrichment set analysis.
RESULTS
A total of 529 low-grade glioma samples and 5 non-tumor brain tissue samples are obtained from the TCGA database. Two hundred forty-seven immune-associated lncRNAs are screened. Cox regression showed that 16 immune-related lncRNAs are associated with low-grade glioma prognosis, and 7 lncRNAs are independent risk factors. Gene set enrichment analysis suggests that these molecules are enriched in extracellular region, sequence-specific DNA binding, neuropeptide signaling pathway, transcriptional misregulation in cancer, cytokine-cytokine receptor interaction, protein digestion and absorption, chemokine signaling pathway, etc. CONCLUSION: The identification of immune-related lncRNA may provide new targets for the research of the molecular mechanisms and treatment of low-grade glioma.
背景
低级别胶质瘤为 I-II 级胶质瘤。免疫疗法是一种很有前途的肿瘤杀伤方法。研究低级别胶质瘤中的免疫分子机制以及发现低级别胶质瘤的新免疫检查点非常重要。
方法
从 TCGA 数据库中下载低级别胶质瘤的基因测序数据和临床数据。通过 Cox 回归鉴定与预后相关的 lncRNA，并通过基因富集集分析寻找其可能的功能。
结果
从 TCGA 数据库中获得了 529 例低级别胶质瘤样本和 5 例非肿瘤脑组织样本。筛选出 247 个与免疫相关的 lncRNA。Cox 回归显示，16 个免疫相关的 lncRNA 与低级别胶质瘤的预后相关，其中 7 个 lncRNA 是独立的危险因素。基因集富集分析表明，这些分子富集在细胞外区域、序列特异性 DNA 结合、神经肽信号通路、癌症转录失调、细胞因子-细胞因子受体相互作用、蛋白质消化和吸收、趋化因子信号通路等。
结论
鉴定免疫相关 lncRNA 可能为低级别胶质瘤的分子机制和治疗研究提供新的靶点。
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