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HEIH 通过调控 STAT3 介导的自噬和糖酵解促进胃癌的恶性进展。

HEIH Promotes Malignant Progression of Gastric Cancer by Regulating STAT3-Mediated Autophagy and Glycolysis.

机构信息

The Medical College of Nanchang University, Nanchang, Jiangxi 330006, China.

Department of Gastrointestinal Medical Oncology, Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, China.

出版信息

Dis Markers. 2022 Jul 27;2022:2634526. doi: 10.1155/2022/2634526. eCollection 2022.

DOI:10.1155/2022/2634526
PMID:36246567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9568361/
Abstract

To study the clinical value of HEIH hyperexpression in gastric cancer and the molecular mechanism of promoting malignant proliferation of gastric cancer cells, qRT-PCR was used to detect the expression of HEIH in gastric cancer and nontumor gastric tissues. HEIH interference sequence was constructed to downregulate HEIH expression in MGC-803 and BGC-823 cell lines. CCK8, clonogenesis, and Transwell assay were used to detect the effects of HEIH on proliferation and invasion of tumor cells. The protein levels of STAT3, p-STAT3, P62, and LC3 were detected by Western blotting. The results showed that HEIH was highly expressed in gastric cancer ( < 0.01). Interference of HEIH expression in MGC-803 and BGC-823 cells reduced the proliferation and invasion of gastric cancer cells, and the results were statistically significant ( < 0.05). HEIH acts as a miRNA sponge for miR-4500. HEIH promotes gastric cancer development by inhibiting miR-4500. STAT3 is a downstream target of miR-4500. HEIH inhibits autophagy and promotes glycolysis. In conclusion, HEIH is highly expressed in gastric cancers. HEIH promotes malignant proliferation and development of gastric cancer cells. HEIH may be a new candidate site for pathological diagnosis and molecular drug therapy for future clinical treatment of gastric cancer.

摘要

为了研究 HEIH 在胃癌中的高表达的临床价值及其促进胃癌细胞恶性增殖的分子机制，我们采用 qRT-PCR 方法检测了胃癌及非肿瘤胃组织中 HEIH 的表达情况。构建了 HEIH 干扰序列，下调了 MGC-803 和 BGC-823 细胞系中 HEIH 的表达。CCK8、集落形成和 Transwell 实验检测了 HEIH 对肿瘤细胞增殖和侵袭的影响。Western blot 检测了 STAT3、p-STAT3、P62 和 LC3 的蛋白水平。结果表明，HEIH 在胃癌中高表达（<0.01）。干扰 MGC-803 和 BGC-823 细胞中 HEIH 的表达，降低了胃癌细胞的增殖和侵袭能力，且结果具有统计学意义（<0.05）。HEIH 作为 miR-4500 的 miRNA 海绵，通过抑制 miR-4500 促进胃癌的发生发展。STAT3 是 miR-4500 的下游靶基因。HEIH 抑制自噬并促进糖酵解。综上所述，HEIH 在胃癌中高表达。HEIH 促进胃癌细胞的恶性增殖和发展。HEIH 可能是未来胃癌临床治疗中病理诊断和分子药物治疗的新候选靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/a238fe6961e5/DM2022-2634526.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/c136bd4ad5f5/DM2022-2634526.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/20ef358efbdc/DM2022-2634526.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/60d8a273fa55/DM2022-2634526.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/8b317c803570/DM2022-2634526.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/6542d1163f3f/DM2022-2634526.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/fc361b1a07b2/DM2022-2634526.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/a238fe6961e5/DM2022-2634526.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/c136bd4ad5f5/DM2022-2634526.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/20ef358efbdc/DM2022-2634526.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/60d8a273fa55/DM2022-2634526.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/8b317c803570/DM2022-2634526.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/6542d1163f3f/DM2022-2634526.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/fc361b1a07b2/DM2022-2634526.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228c/9568361/a238fe6961e5/DM2022-2634526.007.jpg

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