Department of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Sensengasse 2a, 1090, Vienna, Austria.
Austrian Cluster for Tissue Regeneration, Vienna, Austria.
Clin Oral Investig. 2020 Mar;24(3):1205-1215. doi: 10.1007/s00784-019-02984-z. Epub 2019 Aug 16.
The impact of kaolinite on human periodontal cells is yet unknown. The aim of the study was to assess the response of human periodontal cells to kaolinite.
Human periodontal cells were treated with kaolinite at reducing concentrations from 30 to 0.0015 mg/mL and with conditioned medium, which was depleted of kaolinite. Cell viability was evaluated with a resazurin-based toxicity assay, Live-Dead staining, and MTT assay and staining. The pro-angiogenic factors vascular endothelial growth factor (VEGF) and interleukin (IL)-6 and IL-8 were quantified via ELISA in periodontal fibroblasts. L-929, a standard cell-line used for cytotoxicity studies, served as control cell line. Composition of kaolinite was verified using energy-dispersive X-ray spectroscopy.
Kaolinite in suspension but not in conditioned medium impaired cell viability dose-dependently. VEGF, IL-6, and IL-8 production was not substantially modulated by kaolinite or the conditioned medium in periodontal cells.
Overall, kaolinite can decrease cell viability dose-dependently while conditioned medium showed no toxic effect. No pronounced impact of kaolinite on VEGF, IL-6, and IL-8 production was observed. This study provided first insights into the impact of kaolinite on human periodontal cells thereby inferring to the basis for the evaluation of kaolinite as a carrier in regenerative dentistry.
Kaolinite, a clay mineral, is successfully used in medicine due to its favorable properties. Also, applications in conservative dentistry are described. However, the response of oral cells to kaolinite is still unclear. Here, we assessed the impact of kaolinite on human periodontal cells.
高岭石对人牙周细胞的影响尚不清楚。本研究旨在评估人牙周细胞对高岭石的反应。
用浓度从 30 至 0.0015mg/ml 的高岭石和经过条件培养基处理的细胞，处理人牙周细胞。用基于 Resazurin 的毒性测定法、Live-Dead 染色和 MTT 染色和染色评估细胞活力。通过 ELISA 定量测定牙周成纤维细胞中的促血管生成因子血管内皮生长因子（VEGF）、白细胞介素（IL）-6 和 IL-8。L-929，一种用于细胞毒性研究的标准细胞系，用作对照细胞系。使用能量色散 X 射线光谱法验证高岭石的组成。
悬浮液中的高岭石而不是条件培养基会剂量依赖性地损害细胞活力。高岭石或条件培养基对牙周细胞中的 VEGF、IL-6 和 IL-8 产生没有明显的调节作用。
总体而言，高岭石可以剂量依赖性地降低细胞活力，而条件培养基没有显示出毒性作用。没有观察到高岭石对 VEGF、IL-6 和 IL-8 产生明显的影响。本研究首次深入了解高岭石对人牙周细胞的影响，从而为评估高岭石作为再生牙科载体的基础提供了依据。
高岭石是一种粘土矿物，由于其良好的特性，已成功用于医学。此外，还描述了在保守牙科中的应用。然而，口腔细胞对高岭石的反应仍不清楚。在这里，我们评估了高岭石对人牙周细胞的影响。
