Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo, SP, Brazil.
Department of Restorative Dentistry, University of São Paulo (USP), School of Dentistry, São Paulo, SP, Brazil.
BMC Oral Health. 2024 May 25;24(1):607. doi: 10.1186/s12903-024-04373-9.
This study aimed to evaluate dentin wear and biological performance of desensitizing materials.
Seventy bovine root dentin blocks were sectioned. Half of the surface of each specimen was untreated (control) and the other half was immersed in EDTA and treated with the following desensitizing materials: placebo varnish (PLA), fluoride varnish (FLU), sodium fluoride (NaF) varnish + sodium trimetaphosphate (TMP), universal adhesive (SBU), S-PRG varnish (SPRG), biosilicate (BIOS), and amelotin solution (AMTN). After application, the specimens were submitted to an erosive-abrasive challenge and the wear analyzed by optical profilometer. Serial dilutions of extracts obtained from the culture medium containing discs impregnated with those desensitizers were applied on fibroblasts and odontoblasts-like cells cultures. Cytotoxicity and production of total protein (TP) by colorimetric assays were determined after 24 h. Data were statistically analyzed using Kruskal-Wallis, Dunn's, One-way ANOVA and Tukey tests (p ≤ 0.05).
No dentin wear was observed only for SBU. The lowest dentin wear was observed for AMTN and TMP. Cell viability was significantly reduced after treatment with undiluted extracts of PLA, FLU, TMP and SBU in fibroblasts and TMP and SBU in odontoblast-like cells. SPRG, BIOS and AMTN were cytocompatible at all dilutions tested. Considering TP results, no statistical difference was observed among the groups and high levels for TP were observed after TMP and FLU treatments.
Universal adhesive system may protect dentin with opened tubules from wear after challenge. Extracts of adhesive and fluoride varnishes presented cytotoxic mainly on fibroblasts. The enamel protein may be a future alternative to treat dentin with opened tubules because it may cause low wear under erosive-abrasive challenge with low cytotoxic effects.
本研究旨在评估脱敏材料的牙本质磨损和生物学性能。
将 70 个牛根牙本质块进行切片。每个标本的一半表面未经处理（对照），另一半浸入 EDTA 并用以下脱敏材料处理：安慰剂清漆（PLA）、氟化物清漆（FLU）、氟化物清漆+三聚磷酸钠（TMP）、通用粘结剂（SBU）、S-PRG 清漆（SPRG）、生物硅酸钙（BIOS）和牙本质蛋白溶液（AMTN）。应用后，将标本进行侵蚀-磨损挑战，并通过光学轮廓仪分析磨损。从含有浸渍有这些脱敏剂的圆盘的培养基中获得的提取物的连续稀释液应用于成纤维细胞和牙本质样细胞培养物。通过比色法测定 24 小时后细胞毒性和总蛋白（TP）的产生。使用 Kruskal-Wallis、Dunn、One-way ANOVA 和 Tukey 检验（p≤0.05）对数据进行统计分析。
仅 SBU 未观察到牙本质磨损。AMTN 和 TMP 观察到的牙本质磨损最低。在用 PLA、FLU、TMP 和 SBU 的未稀释提取物处理成纤维细胞以及 TMP 和 SBU 处理牙本质样细胞后，细胞活力显著降低。在所有测试的稀释度下，SPRG、BIOS 和 AMTN 均具有细胞相容性。考虑到 TP 结果，各组之间未观察到统计学差异，在用 TMP 和 FLU 处理后观察到 TP 水平较高。
通用粘结剂系统可能会保护经过挑战后开小管的牙本质免受磨损。粘结剂和氟化物清漆的提取物主要对成纤维细胞具有细胞毒性。牙釉蛋白可能是治疗开小管牙本质的未来替代方法，因为它在侵蚀磨损挑战下可能会导致低磨损，同时具有低细胞毒性作用。
