蓝细菌产生的有毒肽的改进纯化方法。
Improved method for purification of toxic peptides produced by cyanobacteria.
作者信息
Harada K, Suzuki M, Dahlem A M, Beasley V R, Carmichael W W, Rinehart K L
机构信息
Faculty of Pharmacy, Meijo University, Nagoya, Japan.
出版信息
Toxicon. 1988;26(5):433-9. doi: 10.1016/0041-0101(88)90182-1.
PMID:3142107
Abstract
The improved method consists of ODS-silica gel extraction, and separation on silica gel and HPLC with UV (238 nm) detector. The method has been successfully applied to the isolation of toxic peptides from the Monroe and M-228 strains of Microcystis aeruginosa. This method reduces toxin extraction and separation time, and so enables a rapid isolation of peptide toxins from cyanobacteria.
摘要
改进后的方法包括反相硅胶萃取，以及在硅胶柱上分离并采用紫外（238纳米）检测器的高效液相色谱法。该方法已成功应用于从铜绿微囊藻的门罗菌株和M-228菌株中分离有毒肽。此方法缩短了毒素萃取和分离时间，从而能够快速从蓝细菌中分离肽毒素。
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