利用反相高效液相色谱法分析和纯化蓝藻中的有毒肽。
Analysis and purification of toxic peptides from cyanobacteria by reversed-phase high-performance liquid chromatography.
作者信息
Harada K, Matsuura K, Suzuki M, Oka H, Watanabe M F, Oishi S, Dahlem A M, Beasley V R, Carmichael W W
机构信息
Faculty of Pharmacy, Meijo University, Nagoya, Japan.
出版信息
J Chromatogr. 1988 Sep 2;448(2):275-83. doi: 10.1016/s0021-9673(01)84589-1.
A simple, rapid and reliable chemical analysis method for microcystins (cyanoginosins) has been studied. Three different mobile phases for high-performance liquid chromatography were selected and optimized. Also the adsorptive powers of three commercially available C18 cartridges were compared and the results successfully applied to the clean up of three of the toxins. Finally a total system for the analysis and isolation of microcystins was established.
研究了一种用于微囊藻毒素（蓝藻毒素）的简单、快速且可靠的化学分析方法。选择并优化了三种不同的高效液相色谱流动相。还比较了三种市售C18柱的吸附能力，并将结果成功应用于三种毒素的净化。最后建立了一个用于分析和分离微囊藻毒素的完整系统。
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