Interdisciplinary Research Center in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Lahore 54000, Pakistan; Department of Physics, COMSATS University Islamabad, Lahore Campus, Lahore 54000, Pakistan.
Interdisciplinary Research Center in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Lahore 54000, Pakistan.
Int J Biol Macromol. 2019 Nov 1;140:401-406. doi: 10.1016/j.ijbiomac.2019.08.095. Epub 2019 Aug 14.
Chitosan/collagen-based hydrogels were studied for their promising role in skin tissue engineering applications due to their unique biocompatibility and biodegradation properties. Amino acids are not only the mean of protein building units but also support endothelial cells proliferation and trigger angiogenesis during wound healing. The purpose of this study was to prepare amino acid based pro-angiogenic materials. Three structurally closed amino acids (AA) (arginine, alanine and phenylalanine) were loaded into chitosan/collagen hydrogels (ACC hydrogels) to study their effect on angiogenesis. In this study the ACC hydrogels were prepared through freeze drying procedure and their angiogenic potential was studied by chorioallantoic membrane assay (CAM assay). FTIR analysis was performed to confirm that there was no chemical change took place in polymeric materials during synthesis procedures. Results revealed that, arginine-loaded hydrogels were the most porous, with more interconnected pores and also the maximum growth of blood vessels were found around and inside the arginine loaded scaffold. The qualitative analysis for blood vessels showed the significant difference between control, chitosan/collagen alanine loaded hydrogel (CH-Ala), chitosan/collagen phenylalanine loaded hydrogel (CH-Phe) and chitosan/collagen arginine loaded hydrogel (CH-Arg) materials. Among these studied materials the CH-Arg was found more capable for angiogenesis.
壳聚糖/胶原基水凝胶由于其独特的生物相容性和可生物降解性，在皮肤组织工程应用中具有广阔的应用前景。氨基酸不仅是蛋白质构建单元的手段，而且在伤口愈合过程中支持内皮细胞增殖并触发血管生成。本研究旨在制备基于氨基酸的促血管生成材料。将三种结构封闭的氨基酸（AA）（精氨酸、丙氨酸和苯丙氨酸）负载到壳聚糖/胶原水凝胶（ACC 水凝胶）中，以研究它们对血管生成的影响。在这项研究中，通过冷冻干燥程序制备了 ACC 水凝胶，并通过鸡胚尿囊膜试验（CAM 试验）研究了它们的促血管生成潜力。傅里叶变换红外光谱（FTIR）分析证实，在合成过程中聚合材料没有发生化学变化。结果表明，负载精氨酸的水凝胶具有最多孔的结构，具有更多的相互连接的孔，并且在负载精氨酸的支架周围和内部发现了最多的血管生长。血管的定性分析表明，在对照、壳聚糖/胶原丙氨酸负载水凝胶（CH-Ala）、壳聚糖/胶原苯丙氨酸负载水凝胶（CH-Phe）和壳聚糖/胶原精氨酸负载水凝胶（CH-Arg）材料之间存在显著差异。在这些研究材料中，CH-Arg 被发现更有利于血管生成。
